CsA对LPS诱导的子宫内膜基质细胞中IL-1β和IL-6表达的影响

Effect of CsA on the Expression of IL-1βand IL-6 in Endometrial Stomas Cells Induced by LPS

本研究旨在探索免疫抑制剂环孢菌素A(CsA)对LPS诱导的子宫内膜基质细胞中促炎症细胞因子白细胞介素1β(IL-1β)和白细胞介素6(IL-6)表达的影响。母兔子宫内膜基质细胞经过体外培养和鉴定后,用1μg/mL脂多糖(lipopolysaccharide,LPS)诱导构建子宫内膜基质细胞炎症模型,0μg/mL LPS处理作对照组。用0、50、100、200和500 ng/mL CsA处理后,分别检测IL-1β和IL-6的表达水平。结果显示,与对照组相比,添加LPS显著诱导IL-1β和IL-6在子宫内膜基质细胞中的表达(P<0.05),CsA对子宫内膜基质细胞IL-1β和IL-6的表达无影响,但50、100、200和500 ng/mL CsA均极显著地抑制1μg/mL LPS诱导的IL-1β和IL-6表达的上调(P<0.01)。因此,在子宫内膜基质细胞中,一定剂量的CsA能够有效抑制LPS诱导的子宫内膜基质细胞的免疫应激。本研究为雌性生殖道遭受革兰氏阴性菌感染引起免疫应激后用CsA治疗的可行性提供了依据,但在临床上的应用及其机制尚需进行深入研究。

The aim of this study was to investigate the effect of immunosuppressant cyclosporine A(CsA)on the expression of proinflammatory cytokines interleukin 1 beta(IL-1β)and interleukin 6(IL-6)in LPS-induced uterine endometrial stromas cells(ESCs).After cultured and identified in vitro,the inflammation model of the ESCs of female rabbits were successfully constructed by 1μg/mL lipopolysaccharide(LPS),and 0μg/mL LPS was used as the control.Then the expression levels of IL-1βand IL-6 were detected after treatment with 0,50,100,200 and 500 ng/mL CsA.The results showed that LPS significantly induced the expression of IL-1βand IL-6 in ESCs(P<0.05).Although CsA had no effect on the expression of IL-1βand IL-6,but 50,100,200 and 500 ng/mL CsA extremely significantly inhibited the upregulation of IL-1βand IL-6 induced by 1μg/mL LPS(P<0.01).It suggested that a certain dose of CsA could effectively inhibit the LPS-induced immune stress in ESCs.This study provided a basis for the feasibility of CsA treatment after the genital tract of female infected by gram-negative bacteria,but its clinical application and mechanism still need to be further studied.