摘要
以三丁酸甘油酯和阿魏酸乙酯为原料,采用酶催化方法合成阿魏酰丁酸酯,通过单因素实验和正交实验研究溶剂体系、脂肪酶种类、溶剂配比、底物(三丁酸甘油酯与阿魏酸乙酯)摩尔比、反应温度、反应时间对阿魏酰丁酸酯转化率的影响,优化反应条件。结果表明:合成阿魏酰丁酸酯的最佳反应条件为脂肪酶Novozym 435为催化剂,酶添加量50 mg/m L,[EMIM][TF2N]-甲苯溶剂体系,[EMIM][TF2N]与甲苯质量体积比1∶1,反应温度50℃,底物摩尔比3∶1,反应时间6 d。在最佳反应条件下,阿魏酰丁酸酯转化率达76.24%。
Ferulyl butyrate was synthesized by enzyme catalysis with glyceryl tributyrate and ethyl ferulate as raw materials.The influences of solvent system,lipase type,solvent ratio,molar ratio of substrate(molar ratio of glyceryl tributyrate to ethyl ferulate),reaction temperature and reaction time on the conversion rate of ferulyl butyrate were studied.The results showed that the optimal preparation conditions were obtained as follows:lipase Novozym 435 as catalyst,lipase dosage 50 mg/m L,[EMIM][TF2 N]-toluene as solvent system,ratio of[EMIM][TF2 N]mass to toluene volume 1∶1,reaction temperature50℃,molar ratio of substrate 3∶1 and reaction time 6 d.Under these conditions,the conversion rate of ferulyl butyrate was 76.24%.
作者
杨宏黎
窦义莲
韩琳娜
肖志刚
阮有志
韩旭
YANG Hongli;DOU Yilian;HAN Linna;XIAO Zhigang;RUAN Youzhi;HAN Xu(College of Grain Science and Technology,Shenyang Normal University,Shenyang 110034,China;Department of Biochemical Engineering,Chaoyang Teachers College,Chaoyang 122000,Liaoning,China;College of Food Science,Northeast Agricultural University,Harbin 150038,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2020年第9期30-33,共4页
China Oils and Fats
基金
博士后面上基金(2017M611343)
辽宁省教育厅一般项目(L201609)
沈阳师范大学创新创业项目(201910166243)
农业攻关及产业化指导计划(2019JH8/10200020)。
