迷迭香脂溶性抗氧化剂提取工艺的优化研究

Optimization of extracting oil-soluble rosemary antioxidants

以经不同方法预处理(蒸馏法去精油和水煮法去精油)后的迷迭香叶为原料,无添加食用油为提取剂,通过单因素试验考察提取温度、料液比、提取时间及提取次数对脂溶性抗氧化剂(鼠尾草酸+鼠尾草酚)得率的影响。在单因素试验的基础上,采用响应面法对提取工艺条件进一步优化。结果表明:水煮法去精油的原料迷迭香脂溶性抗氧化剂的最优提取条件为提取温度61℃、料液比1∶11、提取时间61 min、提取次数5次,在此条件下脂溶性抗氧化剂得率为2.8601%;蒸馏法去精油的原料迷迭香脂溶性抗氧化剂的最优提取条件为提取温度71℃、料液比1∶11、提取时间81min、提取次数4次,在此条件下脂溶性抗氧化剂得率为3.8013%。对比分析表明,蒸馏法去精油的迷迭香脂溶性抗氧化剂得率高于水煮法去精油的。

The rosemary leaves was pretreated by extracting essential oil with steam distillation and boiling method,and then was used to extract oil-soluble antioxidant.The edible oil without antioxidant was used as the extractant,oil-soluble antioxidants(carnosic acid and carnosol)yield was used as evaluation index,the effects of extraction temperature,material-liquid ratio,extraction time and extraction times were studied by single factor experiment.On the basis of single factor experiment,the response surface methodology was used to optimize the extraction conditions.The results showed that the optimal extraction conditions with the rosemary leaves pretreated by extracting essential oil with boling method were obtained as follows:extraction temperature 61℃,material-liquid ratio 1∶11,extraction time 61 min and extraction times 5.Under these conditions,the yield of oil-soluble antioxidants was2.8601%.For the rosemary leaves pretreated by extracting essential oil with steam distillation method,the optimal extraction conditions were obtained as follows:extraction temperature 71℃,material-liquid ratio 1∶11,extraction time 81 min and extraction times 4.Under these conditions,the yield of oil-soluble antioxidants was 3.8013%.The yield of oil-soluble antioxidants of rosemary leaves pretreated by extracting essential oil with steam distillation method was higher than that by extracting essential oil with boiling method.