亲水作用色谱串联质谱结合反相色谱串联质谱的油料磷脂轮廓分析

Phospholipids profile analysis of oilseeds by hydrophilic interaction chromatography-tandem mass spectrometry combined with reversed phase liquid chromatography-tandem mass spectrometry

采用超高效液相色谱串联四极杆-飞行时间质谱(UPLC-Q-TOF-MS)技术,建立了一种亲水作用色谱(HILIC)和反相色谱(RPLC)结合的磷脂轮廓分析方法,并对8种植物油料的磷脂分子种(PE、PC、PI、LPE、LPC)进行了系统分析。结果表明:8种油料的PC和PE分子种组成基本相似;PI和LPC、LPE分子种组成差异显著,油菜籽、长柄扁桃仁和美藤果仁的PI含量均高于大豆,油菜籽中PI组成以16∶0-18∶2和18∶1-18∶2为主,长柄扁桃仁的PI以16∶0-18∶1为主,美藤果仁的PI以16∶0-18∶2和18∶2-18∶2为主。棉籽和亚麻籽的溶血磷脂含量较高,其中棉籽溶血磷脂的脂肪酸组成以亚油酸为主,亚麻籽中则以油酸为主。该检测方法快速、灵敏、准确,能同时分离多种甘油磷脂、溶血磷脂及其同分异构体。

Using ultra high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS)technology,a phospholipids profile analysis method combining with hydrophilic chromatography(HILIC)and reversed phase chromatography(RPLC)was established.The molecular species of the main phospholipids in eight oilseeds were comprehensively separated and detected,including PE,PC,PI,LPE and LPC.The results showed that the composition of PC and PE molecular species was generally similar among the eight oilseeds,but the composition of PI,LPC and LPE molecular species was significantly different.The contents of PI in rapeseed,Amygdalus pedunculata pall,and Plukenetia volubilis Linneo were higher than that in soybean.The main composition of PI in rapeseed was 16∶0-18∶2 and 18∶1-18∶2.In Amygdalus pedunculata pall,the main composition was16∶0-18∶1 and the Plukenetia volubilis Linneo was mainly composed of 16∶0-18∶2 and 18∶2-18∶2.Cottonseed and flaxseed had higher lysophospholipid content and fatty acids were mainly linoleic acid for cottonseed,and oleic acid for flaxseed.The method had the items of rapidness,sensitiveness and accuracy,which could realize the simultaneously separation of various glycerophospholipids,lysophospholipids and their isomers.