黄地老虎成虫对15种植物挥发物的电生理和行为反应

Electrophysiological and Behavioral Responses of Agrotis Segetum Adults to 15 Plant Volatiles

【目的】筛选对黄地老虎Agrotis segetum雌性成虫具有引诱活性的植物挥发物,为黄地老虎食诱剂的研发提供候选物质【方法】利用触角电位仪(EAG)测试黄地老虎雌性成虫对15种植物挥发物的电生理反应,通过风洞试验,评价每种物质对黄地老虎雌性成虫的吸引作用。【结果】黄地老虎雌性成虫触角对4种挥发物(癸烷、对乙基苯乙酮、2-甲基壬烷和3,3-二甲基辛烷)没有明显的EAG反应,对其余11种挥发物(1,2-二乙苯、1,4-二乙苯、丁酸丁酯、4,8-二甲基-1,3,7-壬三烯、桉叶油醇、乙酸叶醇酯、芳樟醇、β-月桂烯、壬醛、罗勒烯和β-蒎烯)的电生理反应明显,且反应程度随着挥发物浓度的增加而增加,在最大测试浓度100μg/mL时产生最强烈的电生理反应。在风洞测试中,黄地老虎雌性成虫对1,2-二乙苯、1,4-二乙苯、丁酸丁酯、癸烷、4,8-二甲基-1,3,7-壬三烯、对乙基苯乙酮、桉叶油醇、乙酸叶醇酯、芳樟醇、β-月桂烯、壬醛、3,3-二甲基辛烷、罗勒烯和β-蒎烯14种挥发物表现出明显的行为趋好。【结论】筛选出对黄地老虎雌性成虫具有电生理和行为吸引活性的植物挥发物组分。

【Objective】The study aims to screen plant volatiles attracting adult females of the cutwormAgrotis Segetum.【Method】An electroantennogram(EAG)was used to test the electrophysiological response of female A.Segetum adults to 15 kinds of plant volatiles,and meanwhile,the attraction effect of each substance to female A.Segetum adults was evaluated by wind tunnel test.【Result】Female adults did not show significant electrophysiological responses to four compounds(Decane,4-Ethylacetophenone,2-methylnonane and Octane,3,3-dimethyl-).However,other 11 volatiles including Benzene,1,2-diethyl-,Benzene,1,4-diethyl-,Butanoic acid,butyl ester,(3E)-4,8-dimethyl-1,3,7-Nonatriene,Eucalyptol,cis-3-Hexenyl acetate,Linalool,β-Myrcene,Nonanal,Ocimene andβ-Pinene evoked significant electrophysiological responses in EAG trials.Moreover,the electrophysiological responses increased with the increasing volatile concentrations,and the strongest response was produced at the maximum test concentration of 100μg/mL.Female adults were also highly attracted by 14 compounds(Benzene,1,2-diethyl-,Benzene,1,4-diethyl-,Butanoic acid,butyl ester,Decane,(3E)-4,8-dimethyl-1,3,7-Nonatriene,4-Ethylacetophenone,Eucalyptol,cis-3-Hexenyl acetate,Linalool,β-Myrcene,Nonanal,Octane,3,3-dimethyl-,Ocimene andβ-Pinene)in wind tunnel trials.【Conclusion】Overall,some plant volatiles evoked electrophysiological and behavioral responses by female A.Segetum are screened,which provides candidate substances for the development of its food attractants in the future.