AP1S1基因在乳腺癌中的表达与临床意义

Expression and clinical significance of AP1S1 gene in breast cancer

目的探究衔接因子相关蛋白复合体1,σ1亚基(AP1S1)基因在乳腺癌中的表达与临床意义。方法采用GEPIA和UALCAN在线数据库中公开的乳腺癌患者手术组织与癌旁组织(正常对照)中AP1S1表达水平的数据,分析AP1S1表达水平与乳腺癌患者临床预后之间的关系。收集在甲乳外科行乳腺根治术或改良根治术并且其临床资料完整的蜡块标本110例,采用免疫组化方法检测AP1S1在乳腺癌组织中的表达。体外实验采用平板克隆、CCK-8与细胞划痕实验分析AP1S1的表达对乳腺癌细胞增殖、转移的影响。结果通过GEPIA与UALCAN数据分析显示乳腺癌组AP1S1表达水平高于正常对照组(P<0.01),AP1S1的表达水平与总体生存期(OS)和无病生存期(DFS)存在关联(P<0.05),并且与乳腺癌患者组织肿瘤分期相关(P<0.05),与年龄、肿瘤分型及淋巴结转移等无相关性。临床病例分析验证了AP1S1表达与乳腺癌肿瘤大小及AJCC分期密切相关(P<0.05),与年龄、淋巴结转移、雌激素受体(ER)状态、孕激素受体(PR)状态、人表皮生长因子受体-2(HER2)状态无相关性(P>0.05)。细胞功能实验结果显示AP1S1基因表达会促进乳腺癌细胞的增殖、克隆形成及迁移能力。结论AP1S1基因在乳腺癌组织中高表达,其表达水平与乳腺癌患者的预后相关;而且AP1S1基因表达能够促进乳腺癌细胞增殖、克隆形成与迁移能力。

Objective To investigate the expression and clinical significance of AP-1 complex subunit sigma-1 A(AP1 S1) gene in breast cancer.Methods The relationship between the expression level of AP1 S1 and the clinical prognosis of breast cancer patients was analyzed using the data in surgical tissues and adjacent tissues of breast cancer patients published in the online databases of GEPIA and UALCAN. 110 cases of wax block specimens with complete clinical data and complete clinical data were collected. The expression of AP1 S1 in breast cancer tissues was detected by immunohistochemistry. In vitro experiments were performed using plate clones, CCK-8 and cell scratch experiments to analyze the effect of AP1 S1 expression on the proliferation and metastasis characteristics of breast cancer cells. Results Through the analysis of GEPIA and UALCAN data, it was found that the expression level of AP1 S1 in breast cancer tissues was significantly higher than that in normal breast tissues(P<0.01). The expression level of AP1 S1 was significantly associated with overall survival(OS) and disease-free survival(DFS)( P<0.05), and it was closely related to the tumor stage of breast cancer patients(P<0.05), and had no obvious correlation with age, tumor classification and lymph node metastasis. Clinical case analysis confirmed that AP1 S1 expression was closely related to breast cancer tumor size and AJCC stage(P<0.05), and had no significant correlation with age, lymph node metastasis, estrogen receptor(ER) status, progesterone receptor(PR) status, and human epidermal growth factor receptor 2(HER2) status(P>0.05). The results of cell function experiments showed that the expression of AP1 S1 gene could promote the proliferation, colony formation and migration ability of breast cancer cells.Conclusion AP1 S1 gene is highly expressed in breast cancer tissues, and its expression level is related to the prognosis of breast cancer patients. Moreover, AP1 S1 gene expression can promote breast cancer cell proliferation, colony formation and migration ability.