类风湿关节炎患者血浆外泌体miR-16-5p和miR-223-3p的表达及临床意义

Expression and clinical significance of miR-16-5p and miR-223-3p in plasma exosomes of patients with rheumatoid arthritis

目的通过检测miR-16-5p及miR-223-3p在类风湿关节炎(RA)患者和正常人血浆外泌体中的表达水平,分析其与RA临床指标间的关系,进一步了解其在RA疾病中的临床价值。方法采用exoE asy Maxi Kit试剂盒提取RA患者和正常人血浆中的外泌体,采用实时荧光定量逆转录聚合酶链式反应技术(RT-qPCR)对外泌体中miR-16-5p和miR-223-3p进行定量检测。结果外泌体miR-16-5p在RA组[1.852 (1.084,2.532)]中的表达高于健康对照组[0.995(0.572,1.798)],差异有统计学意义(P <0.05);但其在RA活动组[1.427(0.950,2.314)]中的表达水平与RA稳定组[1.970 (1.248,2.857)]相当,差异无统计学意义(P>0.05)。外泌体miR-223-3p在RA组[106.611 (46.813,223.841)]的表达水平高于健康对照组[47.586 (8.115,101.347)],在RA活动组[199.615 (104.944,305.090)]中的相对表达水平也高于RA稳定组[53.993 (21.524,107.707)],并且miR-223-3p相对表达水平与患者关节压痛数(TJC)、关节肿胀数(SJC)、基于CRP及ESR水平计算的28个关节疾病活动性评分(DAS28-CRP、DAS28-ESR)均呈正相关(P <0.05)。结论 miR-16-5p和miR-223-3p在RA患者血浆外泌体中异常表达,提示其在RA发病中可能起重要作用。

Objective By detecting the expression levels of miR-16-5 p and miR-223-3 p in the plasma exosomes of rheumatoid arthritis(RA) patients and normal people, the relationship between them and clinical indicators of RA was analyzed to further understand their clinical value in RA diseases. Methods ExoEasy Maxi kit was used to extract exosomes from RA patients and normal human plasma, and miR-16-5 p and miR-223-3 p were quantitatively detected in exosomes using reverse transcription quantitative polymerase chain reaction technology. Results The expression of exosomes miR-16-5 p in the RA group [1.852(1.084, 2.532)] was higher than that in the healthy control group [0.995(0.572, 1.798)], and the difference was statistically significant(P<0.05). However, its expression level in the RA active group [1.427(0.950, 2.314)] was comparable to the RA stable group [1.970(1.248, 2.857)], and the difference was not statistically significant(P> 0.05). The expression level of exosomes miR-223-3 p in the RA group[106.611(46.813, 223.841)] was higher than that in the healthy control group[47.586(8.115, 101.347)], and its expression level in the RA active group [199.615(104.944, 305.090)] was also higher than that in the RA stable group [53.993(21.524, 107.707)](P<0.05). At the same time, the relative expression level of miR-223-3 p was positively correlated with the patients’ TJC, SJC, DAS28-CRP and DAS28-ESR scores(P<0.05). Conclusion The abnormal expression of miR-16-5 p and miR-223-3 p in plasma exosomes of RA patients suggests that they may play an important role in the pathogenesis of RA.