超声辅助酸提取-ICP-MS快速测定水产品中多种重金属

Rapid Detection of Multi-Elements in Aquatic Products Using Ultrasonic-Assisted Acid Extraction by ICP-MS Method

应用超声辅助酸提取-电感耦合等离子体质谱(ICP-MS)技术,建立了快速测定水产品中多种金属(铅、汞、锰、镉、铜、锌、硒)的分析方法。实验中优化了酸浓度、超声温度、超声时间对各种金属提取的影响,结果表明,在20%硝酸浓度、超声水浴温度55 ℃、超声时间20 min的条件下,均能较好地提取水产品大虾和扇贝中多种金属。在最佳实验条件下,实际海产品虾和贝中7种金属加标回收率在85.1%~107.8%,相对标准偏差RSD为1.9%~9.8%,与国标法测试结果相对偏差均在10%以下。超声辅助酸提取-ICP-MS能够快速、准确地检测水产品中上述7种金属含量,可将样品前处理时间缩短至30 min左右。

The method for rapid determination of multi-elements (Pb,Hg,Mn,Cd,Cu,Zn,Se) in aquatic products was established using ultrasonic-assisted acid extraction method by inductively coupled plasma mass spectrometry (ICP-MS).The effects of acid concentration,ultrasonic temperature,and ultrasonic time on the extraction efficiency of various metals were optimized.The results showed that the optimal extraction concentration was 20% nitric acid.Besides,the optimal extraction time and temperature were 20 minutes and 55 ℃ respectively.Under the optimal experimental conditions,the recoveries of seven metals in actual aquatic products were observed in the range of 85.1%~107.8%,the relative standard deviations were between 1.9% and 9.8%.Comparison with the traditional microwave digest method,the relative deviation of the results is less than 10% for two methods.The ultrasonic-assisted acid extraction method was suitable for multi-elements detection in aquatic products and can reduce the sample pretreatment time to about 30 minutes.