血管内皮生长因子过表达骨髓间充质干细胞对支气管肺发育不良的保护作用

Protective effect of VEGF overexpression bone marrow mesenchymal stem cells on bronchopulmonary dysplasia

目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)过表达髓间充质干细胞(bone marrow mesenchymal stem cells,BMSC)对支气管肺发育不良(bronchopulmonary dysplasia,BPD)的保护作用及其机制。方法通过原代细胞分离技术得到BMSCs细胞,并通过免疫荧光技术检测干细胞标志物CD44、CD106的表达,以及构建稳定过表达VEGF的BMSCs(BMSC-VEGF)。通过高氧环境构建BPD幼鼠模型,静脉注射BMSC-VEGF,检测其对幼鼠肺呼吸阻力、肺部发育和重构的影响。结果VEGF可以维持BDP动物模型中肺部组织的增殖活性,改善肺内血管形成以及抑制TGF-β通路活化所致肺组织重构等作用。结论在幼鼠中,过表达VEGF对BPD具有明显的保护作用,VEGF有可能成为治疗BPD的候选治疗手段。

Objective To explore the protective role and mechanism of vascular endothelial growth factor(VEGF)overexpression bone marrow mesenchymal stem cells(BMSC)on bronchopulmonary dysplasia(BPD).Methods BMSCs cells were obtained by primary cell isolation,and the expression of stem cell markers CD44 and CD106 was detected by immunofluorescence technology,and BMSCs(BMSC-VEGF)with stable expression of VEGF were constructed.Then,BPD pups model was constructed through hyper-oxygen environment,and BMSCVEGF was injected intravenously to detect the effect of BMSC-VEGF on lung respiratory resistance,lung development,and reconstruction of pups.Results The results showed that VEGF could maintain the proliferative activity of lung tissue in BDP animal model,prevent the reduction of pulmonary blood vessels,and inhibit the lung tissue reconstruction caused by the activation of TGF-βpathway.Conclusion VEGF has obvious protective effect on BPD in young mice,and VEGF may be a candidate for treatment of BPD.