CCDC92基因 rs11057401单核苷酸多态性与冠心病风险的关系

Relationship between CCDC92 gene rs11057401 SNP and risk of coronary heart disease

目的:探讨卷曲螺旋结构域蛋白(CCDC)92基因rs11057401单核苷酸多态性(SNP)与冠心病(CHD)风险的关系。方法:选择我科732例CHD患者为CHD组,同期734例健康体检者为正常对照组,均抽取外周静脉血,应用基于PCR法的基因探针技术对rs11057401 SNP基因位点进行基因多态性检测,比较两组基因型和等位基因频率,CHD组内不同基因型亚组间的血液指标水平,以及分析CHD的独立危险和保护因素。结果:与正常对照组比较,CHD组年龄≥60岁比例、FPG、TC、LDL-C、糖尿病、高血压比例显著升高,HDL-C、载脂蛋白(Apo)A1、ApoA1/ApoB显著降低,P<0.05或<0.01;rs11057401 SNP的AA基因型频率(14.0%比27.5%)、等位基因A频率(38.8%比51.7%)均显著升高,P均=0.001。多元Logistic回归分析显示rs11057401 AA/AT、糖尿病、高血压、LDL-C和TC均为CHD的独立危险因素(OR=1.002~4.536,P<0.05或<0.01),而HDL-C为独立保护因素(OR=0.917,P=0.032)。CHD组内,AA亚组的FPG水平显著高于TT亚组,而ApoA1水平显著低于TT、AT亚组,P<0.05或<0.01。结论:CCDC92基因rs11057401 SNP的基因多态性与冠心病风险存在关系,对于冠心病的早期筛查与诊治有重要价值。

Objective:To explore relationship between coiled-coil domain containing proteins(CCDC)92 gene rs11057401 single nucleotide polymorphism(SNP)and risk of coronary heart disease(CHD).Methods:A total of 732 CHD patients from our department were regarded as CHD group,another 734 healthy subjects were simultaneously enrolled as normal control group.Peripheral venous blood was taken from all subjects,gene probe technology based on PCR method was used to detect genetic polymorphism of rs11057401 SNP loci.Genotype and allele frequency of two groups,blood index levels of different genotype subgroups of CHD group were compared,and independent risk and protective factors of CHD were analyzed.Results:Compared with normal control group,there were significant rise in percentages of age≥60 years,diabetes mellitus and hypertension,levels of FPG,TC and LDL-C,and significant reductions in levels of HDL-C,apolipoprotein(Apo)A1 and ApoA1/ApoB in CHD group,P<0.05 or<0.01;significant rise in genotype AA frequency(14.0%vs.27.5%)and allele A frequency(38.8%vs.51.7%)of rs11057401 SNP in CHD group,P=0.001 both.Multivariate Logistic regression analysis indicated that rs11057401 AA/AT,diabetes mellitus,hypertension,LDL-C and TC were independent risk factors for CHD(OR=1.002~4.536,P<0.05 or<0.01),while HDL-C was independent protective factor(OR=0.917,P=0.032).In CHD group,FPG level of AA subgroup was significantly higher than that of TT subgroup,while ApoA1 level of AA subgroup was significantly lower than those of TT and AT subgroups,P<0.05 or<0.01.Conclusion:SNP of CCDC92 gene rs11057401 is associated with risk of CHD,which possesses important value for early screening,diagnosis and treatment of CHD.