CAG多肽修饰磁性四氧化三铁纳米颗粒及其对内皮细胞的影响

CAG peptide modified magnetic ferroferric oxide nanoparticles and its effect on endothelial cells

在心血管系统中,内皮细胞对于维持血管环境的稳态具有重要作用,是修复血管受损的关键所在。磁性四氧化三铁纳米颗粒(Fe3O4-NP)具有良好的磁靶向性,在生物材料领域备受关注。但较差的细胞相容性严重限制其在心血管及内皮细胞治疗领域的应用。本研究通过对Fe3O4-NP进行表面改性,将具有良好内皮细胞亲和性的CAG多肽引入到纳米颗粒表面,获得功能化的Fe3O4-CAG颗粒,以提高纳米颗粒的细胞相容性。结果表明,Fe3O4-CAG颗粒分散性得到改善,磁性性能有所下降;可使内皮细胞活性增强,增殖率提高187.4%;在外加磁场作用下,可诱导内皮细胞定向迁移,迁移距离上升33.5%。研究结果说明,通过对Fe3O4-NP表面进行CAG多肽修饰,为其在心血管系统及血管修复中的研究与应用提供了一种新的手段。

In the cardiovascular system,endothelial cells play an important role in maintaining the steady state of the vascular environment and are the key to repairing damaged blood vessels.Magnetic ferroferric oxide nanoparticles(Fe3O4-NP)have good magnetic targeting and attract much attention in the field of biomaterials.However,poor cell compatibility severely limits its application in the field of cardiovascular and endothelial cell therapy.In this study,through surface modification of Fe3O4-NP,CAG peptides with good affinity for endothelial cells were introduced to the surface of nanoparticles to obtain functionalized Fe3O4-CAG nanoparticles so as to improve the cell compatibility of nanoparticles.The results showed that the dispersion of Fe3O4-CAG particles was improved and the magnetic properties were reduced.The activity of endothelial cells could be enhanced and the proliferation rate could be increased by 187.4%.Under the action of an external magnetic field,the endothelial cells could be induced to migrate directionally and the migration distance increased by 33.5%.The results of this study indicate that by modifying CAG polypeptide on the surface of Fe3O4-NP,it provided a new method for its research and application in the cardiovascular system and vascular repair.