四逆汤对肝母细胞瘤细胞生物学行为的影响及机制

Effect and mechanism of Sini decoction on malignant biological function of hepatoblastoma cells

目的探讨四逆汤对肝母细胞瘤细胞(Hep G2细胞)生物学行为的影响及机制。方法常规培养Hep G2细胞并随机分为对照组与四逆汤组,四逆汤组加入终浓度为50 mg/mL的四逆汤水煎液,对照组加入等体积的PBS溶液,继续培养48 h。用MTT实验检测细胞增殖能力,细胞划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力,流式细胞术检测细胞凋亡情况,Western blotting法检测细胞内低氧诱导因子1α(HIF-1α)、蛋白激酶B1(AKT-1)及过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)蛋白表达。结果与对照组比较,四逆汤组Hep G2细胞增殖率低,细胞迁移距离短,向Transwell下室迁移的细胞比例低,细胞凋亡率高,HIF-1α、AKT-1、PGC-1α蛋白相对表达量低(P均<0.05)。结论四逆汤能抑制Hep G2细胞增殖、迁移及侵袭,并促进其凋亡;该作用机制可能与促进细胞内HIF-1α、AKT-1、PGC-1α蛋白降解有关。

Objective To explore the effect and mechanism of Sini decoction on the malignant biological function of hepatoblastoma cells.Methods Hep G2 cells were cultured routinely and randomly divided into the control group and Sini decoction group;the cells in the Sini decoction group were treated with Sini decoction at a concentration of 50 mg/mL,and the control group with an equal volume of PBS solution.The proliferation activity of Hep G2 cells was detected by MTT assay,the ability of cell migration was detected by Scratch test and Transwell test,apoptosis was detected by flow cytometry.The protein levels of HIF-1α,Akt-1,and PGC-1αwere detected by Western blotting.Results Compared with the control group,the growth rate of Hep G2 cells treated with Sini decoction was lower,cell migration distance was shorter,the proportion of cells that migrated to the lower chamber of the Transwell was smaller,and the apoptosis rate was higher(all P<0.05).The expression levels of HIF-1α,Akt-1 and PGC-1αin the Sini decoction group were significantly lower(all P<0.05).Conclusion Sini decoction can effectively inhibit the proliferation,migration and invasion of Hep G2 cells,and promote its apoptosis;the mechanism may be related to the promotion of HIF-1α,AKT-1 and PGC-1αprotein degradation in cells.