高效液相色谱法测定酒石酸泰乐菌素药物中组胺含量

Determination of Histamine in Tylosin Tartrate by HPLC

目的:建立酒石酸泰乐菌素药物中组胺的柱前衍生、液相色谱检测方法。方法:样品经三氯乙酸溶液提取、丹磺酰氯衍生化反应后用高效液相色谱-紫外检测器检测,外标法定量。结果:方法的定量检出限为0.0016 mg/kg,浓度在0.0016~7.4970 mg/L范围内线性良好(R^2=0.9996),添加浓度在0.47μg/g、47.00μg/g和56.00μg/g时,回收率在100%~103%,47μg/g浓度下方法精密度RSD为0.708%。结论:该方法适用于酒石酸泰乐菌素药物中组胺的测定。

Objective:to establish a HPLC method for the determination of histamine in tylosin tartrate.Methods:the samples were extracted by trichloroacetic acid solution,derivatized by dansyl chloride,detected by HPLC-UV detector,and quantified by external standard method.Results:the detection limit of the method was 0.001599 mg/kg,and the linear range was 0.0016~7.4970 mg/L(R^2=0.9996).The recoveries were 100%~103%when the concentration was 0.47μg/g,47.00μg/g and 56.00μg/g.the RSD of the method was 0.708%at the concentration of 47μg/g.Conclusion:the method is suitable for the determination of histamine in tylosin tartrate.