聚普瑞锌治疗大鼠缺血性结肠炎的实验研究

Experimental study on the treatment of ischemic colitis in rats with Polaprezinc

目的探索聚普瑞锌(Polaprezinc,PZ)对缺血性结肠炎(ischemic colitis,IC)大鼠结肠黏膜损伤的修复作用及可能机制。方法40只大鼠随机分为3组:实验组(开腹+激光照射+PZ治疗)24只、对照组(开腹+激光照射)12只、空白组4只。光化学法建立IC大鼠模型,实验组造模后每天给予3000 mg/L的PZ溶液3 ml,余自由进食水。对照组造模后与空白组仅自由进食水。术后第5天处死大鼠,肉眼观察结肠黏膜大体表现,显微镜下进行结肠黏膜损伤程度评分,免疫组化方法评估结肠黏膜组织中HSP70和NF-κB p50的表达。结果实验组大鼠肉眼可见结肠损伤显著低于对照组(16.67%vs 58.33%,P<0.05)。实验组结肠黏膜糜烂、炎症及淋巴细胞浸润均较对照组轻,差异有统计学意义(P<0.05)。实验组结肠损伤评分(1.125±1.262)分显著低于对照组(2.750±1.138)分(P<0.01)。实验组结肠黏膜HSP70表达显著高于对照组(P<0.01),实验组NF-κB p50表达显著低于对照组(P<0.01)。结论PZ能够减轻IC模型大鼠结肠黏膜炎症及糜烂,促进黏膜损伤修复;其机制可能是通过促进HSP70表达,降低NF-κB p50表达进而抑制其活性来实现。

Objective To investigate the effect and mechanism of Polaprezinc(PZ)on injury colon of model rats with ischemic colitis(IC).Methods Total of 40 rats were randomly divided into 3 groups:24 rats in the experimental group(operation+laser+PZ treatment),12 rats in the control group(operation+laser),and 4 rats in the blank group.PZ method was used to establish a IC rat model.The experimental rats were given 3 ml of PZ solution with a concentration of 3000 mg/L every day and then free drinking.The control rats and the blank rats were free to drink water.On the 5 th day post-operation,the rats were sacrificed,the gross appearance of colon mucosa was observed by naked eye,the degree of intestinal mucosa damage was evaluated by microscope,and the expressions of HSP70 and NF-κB p50 in the colon mucosa were evaluated by immunohistochemistry.Results The proportion of colonic injury in the experimental group was significantly lower than that in the control group(16.67%vs 58.33%,P<0.05).The colonic mucosal erosion,inflammation and lymphocyte infiltration in the experimental group were less serious than those in the control group,and the difference was statistically significant(P<0.05).The score of intestinal injury in the experimental group(1.125±1.262)was significantly lower than that in the control group(2.750±1.138)(P<0.01).The expression of HSP70 in intestinal mucosa in the experimental group was significantly higher than that in the control group(P<0.01),and the expression of NF-κB p50 in the experimental group was significantly lower than that in the control group(P<0.01).Conclusion PZ can reduce intestinal mucosal inflammation and erosion in IC model rats and promote the repair of mucosal damage.The mechanism may be by increasing the expression of HSP70,reducing the expression of NF-κB p50 and inhibiting its activity.