响应面优化华腺萼木总黄酮和总多糖的超声波辅助提取工艺

Optimization of Ultrasonic Assisted Extraction of Total Flavonoids and Total Polysaccharides from Mycetia sinensis by Response Surface Methodology

为进一步开发天然甜茶资源,以华腺萼木Mycetia sinensis茎叶为原料,超声波辅助水提法提取其中的总黄酮和总多糖,在单因素试验的基础上用响应面优化华腺萼木总黄酮和总多糖的提取工艺。结果发现,对华腺萼木总黄酮提取影响最大的是乙醇浓度,影响最小的是提取时间;对总多糖提取影响最大的是提取时间,影响最小的是提取温度。最终确定华腺萼木总黄酮提取的最佳工艺为超声波功率300 W,料液比1∶95,乙醇浓度75%,提取时间50 min,此工艺条件下总黄酮得率为2.36%;总多糖的最佳提取工艺为提取时间180 min,超声波功率250 W,提取温度80℃,此工艺条件下总多糖得率为82.77%。本研究采用响应面法对华腺萼木总黄酮和总多糖提取条件进行了优化,可以为华腺萼木今后的资源开发提供依据。

In order to further develop the natural sweet tea resources,the stems and leaves of Mycetia sinensis were used as the raw material to optimize the extraction technology of total flavonoids and total polysaccharides from M.sinensis by ultrasonic assisted water extraction.On the basis of single factor experiment,the extraction process of total flavonoids and total polysaccharides was optimized by response surface methodology.The results showed that the ethanol concentration had the greatest influence on the extraction of total flavonoids from M.sinensis and the extraction time had the least influence on it.Extraction time had the greatest effect on the extraction of total polysaccharides and the extraction temperature had the least effect.Finally,the optimum extraction technology of M.sinensis total flavonoids was determined as follows:Ultrasonic power 300 W,ratio of material and liquid 1∶95,ethanol concentration 75%,ultrasonic time 50 min,and the yield of total flavonoids under this process condition was 2.36%.The optimum extraction technology of M.sinensis total polysaccharide was determined as follows:Ultrasonic time 180 min,ultrasonic power 250 W,temperature 80℃,and the yield of total flavonoids under this process condition was 82.77%.In this study,response surface methodology was used to rationally optimize the extraction conditions of the total flavonoids and total polysaccharides from M.sinensis,which can provide a basis for future resource development of M.sinensis.