血管紧张素Ⅱ上调连接子蛋白43(Cx43)促进人肺动脉平滑肌细胞的增殖和迁移

Up-regulation of connexin 43 (Cx43) by angiotensin Ⅱ promotes the proliferation and migration of human pulmonary artery smooth muscle cells

目的以人肺动脉平滑肌细胞(HPASMC)为研究对象,探讨连接子蛋白43(Cx43)对血管紧张素Ⅱ(AngⅡ)诱导的细胞增殖、迁移的影响。方法体外培养的对数生长期HPASMC,分为对照组、AngⅡ处理组、AngⅡ联合二甲基亚砜(DMSO)处理组及AngⅡ联合坎地沙坦处理组。采用CCK-8法检测HPASMC增殖,TranswellTM小室检测HPASMC的迁移能力,划痕实验检测其迁移能力;Western blot法检测HPASMC的Cx43蛋白及其磷酸化、骨桥蛋白(OPN)、增殖细胞核抗原(PCNA)、SMAD家族成员2(SMAD2)、SMAD3的蛋白水平。结果与对照组相比,AngⅡ处理组OPN、PCNA蛋白表达增加,细胞增殖和迁移能力增强;与AngⅡ处理组相比,AngⅡ联合坎地沙坦处理组细胞的增殖和迁移能力降低。与对照组相比,AngⅡ处理组Cx43蛋白及其磷酸化水平均明显增加,SMAD2、SMAD3的蛋白表达增加,而AngⅡ联合坎地沙坦组各蛋白表达较AngⅡ组均明显降低。结论AngⅡ上调Cx43蛋白的表达促进HPASMC增殖和迁移,可能与激活SMAD2/3信号通路有关。

Objective To explore the role of Cx43 in the proliferation and migration of human pulmonary arterial smooth muscle cells(HPASMCs)induced by angiotensinⅡ(AngⅡ).Methods HPASMCs were cultured in vitro and randomly divided into four groups:control group,AngⅡgroup,AngⅡcombined with DMSO group,and AngⅡcombined with candesartan group,and cells were collected in logarithmic growth phase.Cell viability was detected by CCK-8 assay;the migration ability of HPASMCs were measured by wound-healing and TranswellTM assay.The protein levels of Cx43,osteopontin(OPN),proliferating cell nuclear antigen(PCNA),SMAD2 and SMAD3 in HPASMCs were detected by Western blot analysis.Results Compared with the control group,the expression of OPN and PCNA proteins significantly went up in AngⅡgroup,and the cell proliferation and migration ability increased.The cell proliferation and migration ability of the AngⅡcombined with candesartan group were significantly lower than that in the AngⅡgroup.Compared with the control group,the Cx43 protein and its phosphorylation level increased significantly in the AngⅡgroup,and the protein expression of SMAD2 and SMAD3 increased,while the expression of each protein in the AngⅡcombined with candesartan group was significantly lower than those in the AngⅡgroup.Conclusion AngⅡup-regulates the expression of Cx43 protein to promote the proliferation and migration of HPASMCs,which may be related to the activation of SMAD2/3 signaling pathway.