中华猕猴桃果仁油非饱和脂肪酸(果王素)上调含EH结构域蛋白2(EHD2)抑制肺腺癌小鼠移植瘤生长和转移

Unsaturated fatty acid of Actinidia chinesis planch seed oil(kiwi fruit essence)inhibits growth and metastasis of transplanted tumor in lung adenocarcinoma mice by up-regulating EHD2 expression

目的观察含EH结构域蛋白2(EHD2)在中华猕猴桃果仁油非饱和脂肪酸(果王素)抑制肺腺癌移植瘤生长和转移中的作用。方法按随机数字表法将32只接种Lewis肺腺癌细胞的C57BL/6J小鼠分为对照组、(60、120、240)mg/kg果王素处理组,每组8只。接种后第4天起,对照组小鼠予以9 g/L氯化钠溶液灌胃,果王素处理组小鼠分别给予(60、120、240)mg/kg果王素灌胃。测量移植瘤体积,第24天将小鼠断颈处死。取肺组织观察肿瘤转移情况,分离移植瘤并称量瘤体质量,计算转移抑制率与肿瘤抑制率;实时荧光定量PCR测定EHD2 mRNA水平,Western blot法检测移植瘤组织EHD2蛋白水平,免疫组织化学染色检测移植瘤组织EHD2表达。结果与对照组比较,(60、120、240)mg/kg果王素处理组皮下移植瘤的生长速度、体积、质量和肺转移结节数明显降低,而肿瘤抑制率和肺转移抑制率显著增加,皮下移植瘤组织EHD2的表达及其mRNA和蛋白水平增高。与60 mg/kg果王素处理组相比,(120、240)mg/kg果王素处理组上述指标变化更明显且呈剂量依赖性。结论果王素能上调EHD2表达,抑制肺腺癌小鼠移植瘤的生长和转移。

Objective To observe the role of Eps15 homology domain containing protein 2(EHD2)in the inhibitive effects of unsaturated fatty acid of Actinidia chinesis planch seed oil(kiwi fruit essence)on the growth and metastasis of transplanted tumor in lung adenocarcinoma mice.Methods 32 C57BL/6J mice bearing Lewis lung adenocarcinoma cells were randomly divided into the control group,60,120 and 240 mg/kg kiwi fruit essence treatment groups.Each group included 8 animals.From the fourth day after innoculation,the mice in the control group were intragastrically treated with normal saline,and the mice were intragastrically treated with 60,120 or 240 mg/kg kiwi fruit essence in the corresponding kiwi fruit essence treatment groups.After measuring the volume of transplanted tumors,all mice were sacrificed on day 24,and their pulmonary tissues were then removed to observe tumor metastasis.The transplanted tumors were exfoliated and weighed to calculate the metastasis inhibition rate and tumor inhibition rate.The protein expression level of EHD2 in the transplanted tumors was detected by immunohistochemistry and Western blotting.The mRNA expression level of EHD2 in the transplanted tumors was measured using real-time quantitative PCR.Results Compared with the control group,the growth,volume,quality and number of lung metastasis nodules of the subcutaneous transplanted tumors significantly decreased,and tumor inhibition rates and metastasis inhibition rates increased in 60,120,240 mg/kg kiwi fruit essence treatment groups.The protein and mRNA level of EHD2 in the subcutaneous transplanted tumors went up.Compared with the 60 mg/kg kiwi fruit essence treatment group,the above indicators were significantly improved in 120 and 240 mg/kg kiwi fruit essence treatment groups in a dose-dependent manner.Conclusion Kiwi fruit essence can up-regulate EHD2 expression,thereby inhibiting the growth and metastasis of lung adenocarcinoma transplantation tumor in mice.