温通汤对动脉硬化性闭塞症小鼠模型血浆一氧化氮和内皮素的影响

Effect of Wen Tong Tang Decoction on Nitric Oxide and Endothelin in Arteriosclerosis Obliterans Mice Modle

目的 :通过观察中药温通汤对动脉硬化性闭塞症 (ASO)小鼠模型血浆一氧化氮 (NO)和内皮素 (ET)的影响 ,试图探讨ASO的发病机制以及中药的作用机理。方法 :NO、ET检测组各取小鼠30只 ,随机分为 3组 ,对照组喂普通饲料 ,模型组和治疗组制备ASO动物模型。模型组和对照组应用0 .9%NS 0 .2ml/ 10g ;治疗组应用温通汤水煎剂 0 .2ml / 10g。每日一次 ,每 6日为一疗程 ,疗程结束后全部处死动物 ,检测血浆中NO、ET含量。结果 :温通汤有明显的升高NO、降低ET的作用 ,治疗组与模型组相比有显著差异 (P <0 .0 1) ,与对照组相比无明显差异 (P >0 .0 5 )。结论 :温通汤能通过对血浆ET、NO平衡的调节 ,有效的预防脂质在动脉内膜的沉积 ,从而起到治疗ASO的目的。

Objective: To observe the Effect of Wen Tong Tang Decoction on Nitric Oxide,Endothelin in Arteriosclerosis Obliterans Mice Modle,in order to discuss the structure of treatment and the effect of traditional chinese medicine.Methods: Every group included 30 mice,and these mice were divided into three parts in random.The mice in model group and treatment group were induced by special food and methods,and contral group used general food,then the contral group and model group used 0.9%NS0.2 ml/10 g,and treatment group used Wen Tong Tang Decoction.Finally,observed Nitric Oxide and Endothel. Results: Wen Tong Tang Decoction could high the content of Nitric Oxide and low the one of Endothel .The total response rate between model groups and treatment group was obviously different,P <0.01. Conclusion: Through adjust the balance of Nitric Oxide and Endothelin, Wen Tong Tang Decoction was effective in the treatment of Arteriosclerosis Obliterans.