电针干预促进大鼠肩袖损伤修复和运动功能康复

Electro-acupuncture promotes repair of rotator cuff injury in rats

目的观测电针治疗对肩袖损伤模型大鼠的肌腱愈合和功能恢复的治疗作用,检测关节液介素-1β(IL-1β)、IL-6、α肿瘤坏死因子(TNF-α)等3种炎症因子表达以及肩袖生物力学的影响,探讨电针治疗肩袖损伤的疗效及机制。方法选取90只SD大鼠,随机分为电针组、对照组及空白组各30例,其中电针组和对照组行肩袖损伤造模手术,空白组不做处理。术后电针组给予电针干预,空白组与对照组不予干预,分别于造模术后2、4、8周测定各组大鼠的右前足足底热缩足潜伏期(TWL),关节液IL-1β、IL-6、TNF-α含量以及肩袖的最大拉力负荷。结果术后2、4、8周,对照组TWL值均低于空白组,均高于对照组(P<0.05);在3个观测时间点,对照组IL-1β、IL-6和TNF-α含量均高于空白组,电针组的IL-1β、IL-6和TNF-α含量均低于对照组(P<0.05);在造模术后2周时,电针组与对照组最大载荷均小于空白组(P<0.05)。术后4、8周时,电针干预组的最大拉力载荷高于对照组(P<0.05)。结论电针疗法既能有效降低炎症因子的表达以缓解疼痛,又能促进损伤组织的修复以提高肩袖的生物力学性能,是肩袖损伤的有效治疗方法。

Objective To observe the therapeutic effect of electro-acupuncture on tendon healing and functional recovery of rotator cuff injury in rats and explore the therapeutic mechanism of electro-acupuncture.Methods Ninety SD rats were randomly divided into electro-acupuncture group,model group and blank control group,and models of rotator cuff injury were established in the former two groups.The rats in electro-acupuncture group was treated with electro-acupuncture after the operation,and those in the other two groups received no treatment.The right forefoot thermal withdrawal latency(TWL),the contents of IL-1β,IL-6 and TNF-αin the synovial fluid and the maximum tension load of supraspinatus tendon were measured at 2,4 and 8 weeks after the operation.Results TWL in the model group was significantly lower than that in the blank control group and electro-acupuncture group at 2,4 and 8 weeks after the operation(P<0.05).At all these time points,the contents of IL-1β,IL-6 and TNF-αin the synovial fluid were significantly higher in the model group than in the blank control group and electro-acupuncture group(P<0.05).At 2 weeks,the maximum load in electro-acupuncture group and model group were significantly lower than that in the blank control group(P<0.05).At 4 and 8 weeks after the operation,the maximum pull load was significantly greater in electro-acupuncture group than in the model group(P<0.05).Conclusion Electro-acupuncture treatment not only effectively reduces the expression of inflammatory factors to relieve pain,but also promotes the repair of damaged tissue to improve the biomechanical properties of rotator cuff in the rat models.