赭曲霉毒素A量子点免疫荧光探针的制备研究

Preparation of Ochratoxin A Quantum Dots Immunofluorescence Probe

本文利用水热合成法合成了由巯基丙酸包覆的CdTe量子点,以EDC为活化剂将CdTe量子点与赭曲霉毒素A(OTA)单克隆抗体偶联,制备了OTA荧光探针,通过紫外吸收光谱、荧光光谱及酶联免疫吸附试验对偶联产物进行了鉴定,证明合成的荧光探针具有良好的生物活性和荧光特性,可用于OTA快速检测方法的建立。

In this thesis,CdTe quantum dots coated with mercaptopropionic acid were synthesized by hydrothermal synthesis,and CdTe quantum dots were coupled with OTA monoclonal antibody using EDC as activator to prepare OTA fluorescent probes.The coupled products were identified by spectroscopy,and enzyme-linked immunosorbent assay.The synthesized fluorescent probes have good biological activity and fluorescence characteristics and can be used for the establishment of rapid detection methods for OTA.