不同培养条件下杏鲍菇对硒的耐受性与硒形态分析

Selenium Tolerance of Pleurotus eryngii Under Different Culture Conditions and Selenium Morphology Analysis

为了明确不同培养条件下杏鲍菇对外源硒的耐受性,分别在固体培养基、液体发酵培养基和栽培基质中添加不同量的亚硒酸钠,并检测杏鲍菇子实体中富集硒的存在形态。结果表明:固体培养条件下,硒浓度低于160 mg/L时,对菌丝的生长没有显著影响;液体培养条件下,4 mg/L的硒元素即可对杏鲍菇菌丝体的生长产生显著抑制;栽培模式下,基质中补充10~600 mg/kg的硒元素,不会影响杏鲍菇菌丝体的生长,且子实体中的硒含量会随基质中硒浓度的增加而增加。子实体硒形态分析表明,富集硒以硒代蛋氨酸、甲基硒代半胱氨酸、硒代胱氨酸和亚硒酸盐[Se(IV)]四种形式存在,其中硒代蛋氨酸是硒与氨基酸的主要结合形式。

To clarify the tolerance of exogenous selenium to Pleurotus eryngii under different culture conditions,different concentrations of sodium selenite were added to the solid medium,liquid fermentation medium and cultivation substrate,respectively.The tolerance to selenium under different culture conditions were compared,and selenium species in P.eryngii fruiting bodies were also detected.The results showed that selenium concentration,which was less than 160 mg/L,had no significant effect on the growth of mycelia under solid culture condition,while under the condition of liquid culture,4 mg/L of selenium could significantly inhibit the growth of mycelium.In the cultivation mode,10~600 mg/kg of selenium in the cultivation substrate did not affect the growth of mycelia,and the selenium content in the fruiting body increased with the increase of selenium concentration in the cultivation substrate.Selenium speciation analysis of fruiting bodies showed that selenium enriched was in the form of selenomethionine,methylselenocysteine,selenocystine and selenite,among which,selenomethionine was the main form of combination of selenium and amino acids.