盐酸四环素浸泡与投喂标记鲢的效果比较及其浸泡标记对肝脏生理指标的影响

EFFECTIVENESS COMPARISON OF TETRACYCLINE IMMERSING AND FEEDING TO MARK SILVER CARP(HYPOPHTHALMICHTHYS MOLITRIX)AND PHYSIOLOGICAL IMPACTS ON LIVER

本研究以淡水天然水体主要放流鱼类鲢鱼种为实验材料,采用盐酸四环素(TCH)浸泡和药饵投喂两种方式对鲢进行荧光标记,比较这两种TCH处理方法对鲢微耳石的标记效果;测定150、250和350mg/L的浓度条件下浸泡24h鲢血液转氨酶以及肝脏氧化损伤和抗氧化指标的变化。结果显示,TCH浸泡处理比投喂处理对耳石的标记效果更好,在150—350mg/L浓度范围内,浓度越高标记效果越好;TCH浸泡处理24h会引起鲢血清的谷草转氨酶(AST)、谷丙转氨酶(ALT)活性在处理后1d显著升高,2d后降低到对照水平。肝脏氧化损伤指标丙二醛(MDA)水平,抗氧化酶超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均呈现先快速极显著升高,然后下降直至恢复正常水平。表明TCH浸泡对鲢肝脏造成了短暂的功能异常和氧化应激胁迫,但在2—5d后即可恢复正常,TCH浸泡方法是鲢标记放流有效、安全、可靠的方法。本研究结果可为鲢的增殖放流、跟踪监测和效果评估工作提供技术支撑,为其他鱼类的标记放流提供参考。

Silver carp(Hypophthalmichthys molitrix)were treated with tetracycline hydrochloride(TCH)by immersing and feeding for fluorescence labeling.The labeling effects of TCH treatments by immersing and feeding on lapillus of the silver carp were compared.Variations of the blood transaminase,liver oxidative damage,and antioxidant index of the silver carp were measured after 24h immersing at concentrations of 150,250,and 350mg/L.Results show that the effect of TCH immersing was better than that of feeding.In the concentration range of 150—350mg/L,the higher the concentration,the better the labeling effect.The activities of aspartate transaminase(AST)and alanine aminotransferase(ALT)in the serum increased significantly after one day of TCH treatment,and decreased to the level of the control after two days.In addition,the levels of malondialdehyde(MDA),superoxide dismutase(SOD),and catalase(CAT)in liver oxidative damage index increased rapidly and significantly at first,then decreased to normal level.Results show that the TCH immersing resulted in temporary dysfunction and oxidative stress in the liver,but the situation could return to normal after 2—5 days.Overall,the TCH immersing method was an effective,safe,and reliable method for labeling silver carp.The results of this study provides technical support for the stock enhancement and releasing,monitoring and evaluation of silver carp,and reference for other fish marker releasing.