6-DMAP诱导黄河口近江牡蛎(Crassostrea ariakensis)三倍体的研究

STUDY ON 6-DMAP INDUCED TRIPLOID OF CRASSOSTREA ARIAKENSIS FROM HUANGHE RIVER ESTUARY

用6-二甲基氨基嘌呤(6-DMAP)进行了黄河口近江牡蛎(Crassostreaariakensis)的三倍体诱导,以卵裂率、孵化率、诱导三倍体率和三倍体诱导组幼虫的生长率、存活率、三倍体率为指标,研究了诱导浓度、起始诱导时间、诱导持续时间三个因素对黄河口近江牡蛎三倍体的诱导效果。结果表明,三因素对近江牡蛎三倍体诱导效果的影响程度为起始诱导时间>诱导持续时间>诱导浓度。温度25℃,诱导浓度为300-450μmol/L,起始诱导时间为受精后14min,诱导持续时间为15min时,诱导三倍体率可达51.39%-71.67%,卵裂率、孵化率、幼虫壳高日平均生长率和幼虫平均存活率分别能达到65.16%-81.57%、61.25%-86.78%、8.76-9.88μm/d、69.16%-73.29%,眼点幼虫时期三倍体率达53.33%-62.16%。本研究为黄河口近江牡蛎的三倍体诱导提供了理论依据。

To obtain efficient induction method of Crassostrea ariakensis by 6-DMAP,we conducted triploid induction of C.ariakensis under the factors of induction concentration,initial induction time,and induction duration.Samples of C.ariakensis in age of 4—5 years were collected from Dongying City,Shandong,near the Huanghe(Yellow)River mouth.The cleavage rate,hatching rate,triploid rate,and condition of triploid larval population in different induction combinations were studied.Results show that the degree of influence of the three factors on triploid induction was in the order of initial induction time>induction duration>induction concentration.At 25℃,the best induction effect was obtained under the induction concentration of 300—450μmol/L,the induction of 14min after fertilization,and the duration of 15min.The induction rate of triploid was 51.39%—71.67%,the cleavage rate,hatching rate,shell height mean daily growth and mean survival rate of induced triploid larvae were 65.16%—81.57%,61.25%—86.78%,8.76—9.88μm/d,69.16%—73.29%,respectively,and the triploid rate of eye-spot larvae reached 53.33%—62.16%.This study provides a theoretical basis for triploid induction of C.ariakensis in Huanghe(Yellow)River mouth region.