大蒜素对口腔鳞状细胞癌治疗作用的研究

Therapeutic effect of Allicin on oral squamous cell carcinoma

目的通过体外实验探讨大蒜素对口腔鳞癌的治疗作用,并运用网络药理学方法分析其可能的机制。方法通过CCK-8方法、划痕实验及Transwell实验在体外检测大蒜素对SCC-15细胞增殖活性、迁移能力及侵袭活力的影响。获取口腔鳞癌致病基因数据集,根据大蒜素的2D和3D结构预测潜在作用靶基因,构建蛋白互作网络并分析富集网络中的KEGG通路及GO功能。结果大蒜素浓度在200μmol/L以上时可明显抑制SCC-15细胞增殖,且抑制作用随时间及浓度增高而增大(P<0.01)。筛选出的大蒜素潜在靶基因中,有12个基因与口腔鳞癌靶致病基因相交集。KEGG分析揭示交集与细胞周期、p53信号通路等有关。GO分析提示交集对口腔鳞癌细胞的生物进程、分子功能及细胞组分有多重影响。结论大蒜素可能通过多靶点影响多信号通路,从而对口腔鳞癌细胞起到治疗作用。

Objective To investigate the therapeutic effect of Allicin on oral squamous cell carcinoma by in vitro studies,and to analyze the possible mechanism by network pharmacology.Methods The effects of Allicin on the proliferation,migration and invasion of SCC-15 cells were detected by CCK-8 method,scratch test and Transwell test.According to the 2D and 3D structure of Allicin,the potential target genes of Allicin were predicted.The possible pathogenic genes of OSCC were obtained by GEO chip,and the protein interaction network was constructed.The KEGG pathway and GO function in the enriched network were analyzed by bioinformatics method.Results When the concentration of Allicin was over 200μmol/L,the proliferation of SCC-15 cells was significantly inhibited,and the inhibition increased with the increase of time and concentration(P<0.01).Among 104 potential target genes of Allicin,12 genes intersected with oral squamous cell carcinoma target genes.KEGG analysis revealed that the intersection was related to cell cycle,p53 signaling pathway and platinum resistance.GO analysis indicated that intersection had multiple effects on biological process,molecular function and cell components of OSCC cells.Conclusion Allicin may play a therapeutic role in oral squamous cell carcinoma cells by affecting multiple signal pathways through multiple targets.