两种灭菌方法对离体牙灭菌效果及粘接强度影响的研究

Effects of two sterilization methods on sterilization efficacy and bonding strength of extracted human teeth

目的检测高温高压蒸汽灭菌法和过氧化氢低温等离子体灭菌法对离体牙的灭菌效果和对牙本质粘接强度的影响。方法收集因正畸拔除的前磨牙40颗,随机分为两组,每组20颗,分别使用高温高压蒸汽灭菌法和过氧化氢低温等离子体灭菌技术处理离体牙。另取10颗新鲜拔除的前磨牙作为剪切粘接强度测试的阳性对照。以营养琼脂培养法评价灭菌效果,以自酸蚀粘接剂可乐丽SE Bond粘接复合树脂于经灭菌处理的离体牙和新鲜拔除牙齿的牙本质面,行剪切力实验,计算剪切粘接强度,并行统计学分析。结果两种灭菌方法处理后的离体牙,经营养琼脂培养法检验均显示灭菌合格。剪切粘接强度分别为:高温高压组((47.66±1.22)MPa)和低温等离子体组((36.88±2.61)MPa)的剪切粘接强度均低于新鲜离体牙组((64.37±4.88)MPa),P<0.05,且低温等离子体组低于高温高压组(P<0.05)。结论高温高压蒸汽灭菌法和过氧化氢低温等离子体灭菌技术可以被用于离体牙的消毒灭菌,但均导致牙本质粘接强度下降。

Objective To investigate the effects of steam autoclave and hydrogen peroxide plasma sterilization technology(HPPST)on extracted human teeth and the influence of sterilization methods on bond strength of dentin adhesive system.Methods Forty extracted human premolars were collected and divided evenly into two groups randomly:steam autoclave group and HPPST group.Another 10 freshly extracted teeth without sterilization were used as positive control for the bond strength test.After treated by the two sterilization methods respectively,the effects of sterilization were determined by agar plate culture.Composite resins were bonded to the dentins by Clearfil SE Bond.Then the bond strength was detected by shear force experiment and statistical analysis was conducted.Results After treated by the two sterilization methods respectively,all the samples were effectively sterilized determined by agar plate culture.Compared with the freshly extracted teeth((64.37±4.88)MPa),the sterilized teeth((47.66±1.22)MPa)had significantly lower bond strength with the HPPST group((36.88±2.61)MPa)being the lowest.Conclusion Both steam autoclave and hydrogen peroxide plasma sterilization technology are suitable to sterilize extracted human teeth but result in significantly lower bond strength.