长链非编码RNA SNHG1在胆管癌中的表达与临床特征及预后的相关性分析

Expression of LncRNA SNHG1 in cholangiocarcinoma and its correlation analysis of clinical characteristics of cholangiocarcinoma

目的分析肝门部胆管癌病人长链非编码RNA(LncRNA)SNHG1表达水平及其与临床特征的相关性。方法肝门部胆管癌病人36例,利用实时定量PCR检测其肿瘤及对应癌旁组织中LncRNA SNHG1表达水平,根据其表达水平分为高表达与低表达组,分析影响LncRNA SNHG1表达水平的因素及其与病人生存期的关系。结果36例肝门部胆管癌肿瘤组织中LncRNA SNHG1表达水平高于癌旁组织,差异有统计学意义(P<0.05)。淋巴结转移是LncRNA SNHG1表达水平的影响因素,淋巴结转移的病人LncRNA SNHG1低表达组比率高于高表达组(P<0.05)。年龄、血浆CA19-9表达水平和LncRNA SNHG1表达水平均是影响胆管癌病人预后的独立因素(P<0.05)。结论LncRNA SNHG1表达水平与肝门部胆管癌转移有一定相关性,可作为判断胆管癌病人预后的独立危险因素,也可作为肝门部胆管癌综合治疗的潜质靶点。

Objective To detect the role of LncRNA SNHG1 in human cholangiocarcinoma and explore its correlation with clinical characteristics of cholangiocarcinoma.Methods We detected the expression of LncRNA SNHG1 in 36 human cholangiocarcinoma tissues and normal bile duct tissues by real-time PCR.And we divided the cholangiocarcinoma patients into high level SNHG1 expression group and low level SNHG1 expression group.And we analyzed the factors influencing the expression level of LncRNA SNHG1 and their relationship with survival of cholangiocarcinoma patients.Results The expression of LncRNA SNHG1 in human cholangiocarcinoma tissues was significantly higher than the normal bile duct tissues(P<0.05).The lymph node metastasis was the influencing factor of LncRNA SNHG1 expression level.The ratio of low expression level of LncRNA SNHG1 was higher than that of high expression level of LncRNA SNHG1 in patients with lymph node metastasis(P<0.05).The age,CA19-9 expression level and expression level of LncRNA SNHG1 were an independent factor influencing the clinical prognosis of patients with cholangiocarcinoma(P<0.05).Conclusion The expression of LncRNA SNHG1 is related to the distant metastasis of cholangiocarcinoma,which could be an independent risk factor for prognostic judgement and potential therapeutic target.