胆固醇对HepG2细胞脂代谢通路的影响

Effect of cholesterol on lipid metabolism pathway in HepG2 cells

肝癌是全球死亡率最高的恶性肿瘤,胆固醇可能参与其中。本研究在体外将胆固醇作用于人肝癌细胞系HepG2细胞,结果显示胆固醇能显著降低HepG2的细胞活力,且随着浓度的增加细胞活力逐渐降低。脂质代谢对肝癌细胞的生长具有重要的作用,因此本研究进一步采用qRT-PCR法检测了胆固醇对HepG2细胞中脂代谢通路中关键基因表达的影响。结果显示,胆固醇显著降低了HepG2细胞中FASN(脂肪酸合酶)基因、SREBP-1c(固醇调节元件结合蛋白-1c)基因和ACC1(CoA羧化酶1)基因的mRNA水平,同时抑制了细胞中SCD1(硬脂酰辅酶A去饱和酶1)基因的表达,而对CPT1(肉碱棕榈酰转移酶1)基因的表达无明显影响。

Liver cancer is the deadliest malignancy in the world,and cholesterol may be involved.In this study,cholesterol was applied to human hepatoma cell line HepG2 in vitro,and the results showed that cholesterol significantly reduced the cell activity of HepG2 in a concentration dependent manner.Lipid metabolism in liver cancer cells plays an important role in cell growth.Therefore,we further used qRT-PCR to detect the effect of cholesterol on the expression of key genes in the lipid metabolism pathway of HepG2 cells.Results showed that the cholesterol significantly reduced the mRNA levels of FASN(Fatty acid synthase),SREBP-1c(Sterol regulatory element-binding protein 1C)and ACC1(Acetyl-CoA carboxylase 1),and also suppressed the expression of SCD1(Stearoyl-CoA desaturase 1),with no influence on CPT1(Carnitine palmitoyltransferase 1).