NF-κB活化对THP-1源性泡沫细胞胆固醇流出与炎症因子表达的影响及右归丸的干预机制研究

Effect of nuclear factor-kappa B activation on cholesterol efflux and expression of inflammatory factors in THP-1-derived foam cells and the intervention mechanism of Yougui pills

目的:通过脂多糖(LPS)干预单核细胞(THP-1)源性泡沫细胞制备高胆固醇模型,探讨右归丸含药血清对THP-1源性泡沫细胞胆固醇流出与炎症因子表达的影响。方法:利用LPS培养THP-1源性泡沫细胞诱导24h制备高胆固醇细胞模型,给予右归丸含药血清小、中、大剂量干预。通过比色法检测干预后的THP-1源性泡沫细胞内总胆固醇(TC)、游离胆固醇(FC)和胆固醇酯(CE)的含量;酶联免疫吸附法测定细胞内的细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)的含量;逆转录-聚合酶链反应法检测细胞NF-κB p65、SREBP1c、ABCA1mRNA的含量和蛋白表达。结果:10 ng/ml LPS培养THP-1源性泡沫细胞成功复制了高胆固醇细胞模型。右归丸大、中、小剂量组THP-1源性泡沫细胞内TC、FC、CE、IL-1β、IL-6、TNF-α、NF-κB p65、SREBP1c、ABCA1mRNA含量以及NF-κBp65、SREBP1c、ABCA1mRNA蛋白表达水平与正常组比较,差异均有统计学意义(P<0.05或P<0.01)。结论:右归丸可能是通过抑制NF-κB p65和SREBP1c基因表达而调节血脂,同时通过抑制炎症因子的释放来预防动脉粥样硬化,为临床预防和治疗动脉粥样硬化提供了新的靶点。

Objective:To establish a model of high cholesterol by lipopolysaccharide(LPS)intervention of THP-1-derived foam cells,and to investigate the effect of serum containing Yougui pills on cholesterol efflux and expression of inflammatory factors in THP-1-derived foam cells.Methods:THP-1-derived foam cells were cultured with LPS for 24 hours to establish a model of high cholesterol,and then the cells were given the intervention of low-,middle-,and high-dose serum containing Yougui pills.Colorimetry was used to measure the content of total cholesterol(TC),free cholesterol(FC),and cholesteryl ester(CE)in THP-1-derived foam cells after intervention;ELISA was used to measure the levels of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in cells;RT-PCR was used to measure the m RNA expression of NF-κB p65,SREBP1 c,and ABCA1 in cells.Results:The model of high cholesterol was successfully established by the intervention of THP-1-derived foam cells with 10 ng/ml LPS.There were significant differences in TC,FC,CE,IL-1β,IL-6,TNF-α,and m RNA and protein expression of NF-κB p65,SREBP1 c,and ABCA1 between the normal group and the low-,middle-,and high-dose serum containing Yougui pills groups(P<0.05 or P<0.01).Conclusion:Yougui pills regulate blood lipids by inhibiting the m RNA expression of NF-κB p65 and SREBP1 c and prevent atherosclerosis by inhibiting the release of inflammatory factors,which provides new targets for the prevention and treatment of atherosclerosis in clinical practice.