不同光强下种植的紫花地丁对清热作用的影响

Effects of light intensity on cleaning away heat property of Viola yedoensis

研究光照强度对紫花地丁清热药效的影响。采用角叉菜胶对ICR小鼠进行急性炎症造模,应用热红外成像技术和称重法比较不同光强条件下(100%全光照组、80%全光照组、50%全光照组、35%全光照组、5%全光照组)生长的紫花地丁对造模前后小鼠局部体温、热辐射、组织肿胀度,酶联免疫法检测小鼠肝脏能量代谢相关酶活性,同时采用MTT细胞增殖法比较不同光强下紫花地丁对人肺癌细胞(A549细胞)增殖的影响。结果表明,造模前,紫花地丁组小鼠体温除5%全光照组外,其余4组均显著低于空白组(P<0.05),体温下降趋势与光强呈正比;紫花地丁组均能减轻造模后小鼠足部肿胀度、降低肝脏组织SDH酶活性[其中100%全光照组和80%全光照组显著低于模型组(P<0.05)],抑制和减轻程度与光强呈正相关,但对肝脏组织组织Na+-K+-ATPase和Ca^2+-Mg^2+-ATPase活性影响不显著;紫花地丁组小鼠足部组织IL-1β,IL-6,TNF-α,PGE2均显著低于模型组,其中80%全光照组IL-1β,IL-6,PGE2含量最低,各光照组间TNF-α无显著差异。紫花地丁水提物对A549细胞的抑制率随着紫花地丁种植光强的增加呈升高趋势,100%光强下紫花地丁对A549细胞增殖的抑制作用显著高于其他组别(P<0.05)。综上,不同光强下种植的紫花地丁均有清热消炎作用,且种植光强与清热药效成正相关,符合"光照-寒热药性"假说,紫花地丁作为中药基原使用应在全光照下种植。

The current study was conducted to explore the effects of light intensity in cultivating environment on the cleaning away heat property of Viola yedoensis.In the present study,we established the acute inflammation model of ICR mice by injecting carrageenan.We compared the effects of V.yedoensis grown under different light intensities(100%,80%,50%,35%and 5%of full sunlight)on mice body temperature,thermal radiation and the swelling degree of foot tissue before and after modeling observing by thermal infrared imaging technique and weighing method.The changes of energy metabolism related enzymes in liver were detected by enzyme-linked immunosorbent assay(ELISA).In addition,the effects of V.yedoensis grown under different light intensities on human lung cancer cell A549 proliferation were explored with MTT method.The results showed that the body temperature of all groups of mice in V.yedoensis group were significantly lower than that of the blank group,except 5%full sunlight group,and the body temperature declined in positive proportion to light intensity.V.yedoensis group could alleviate foot swelling,reduce SDH activity in liver tissue(especially 100%full sunlight group and 80%full sunlight group were significantly lower than model group),and the degree of alleviating and reducing was positively correlated with light intensity.There was no significant difference in the activity of Na+-K+-ATPase and Ca^2+-Mg^2+-ATPase in liver tissue among treatments.The contents of IL-1β,IL-6,TNF-α,PGE2 in foot tissue of mice in V.yedoensis groups were significantly lower than those in model group.Among them,the lowest levels of IL-1β,IL-6,TNF-α,PGE2 were found in 80%full sunlight group,and there was no significant difference in TNF-αamong different groups.The effects of V.yedoensis aqueous extract on A549 cell line proliferation inhibition rate increased with the light intensities of V.yedoensis cultivating environment.And the effects of V.yedoensis grown under 100%of full sunlight showed significantly higher A549 cell line proliferation inhibition rate compared with other groups(P<0.05).In summary,the light intensity of V.yedoensis cultivating environment is positively correlated with the cleaning away heat property of V.yedoensis,which conforms to the"light-cold and heat property"hypothesis,The V.yedoensis should be planted under full light according.