摘要
目的:探讨山茱萸提取物对慢性肾功能衰竭(CRF)大鼠的肾保护作用及对氧化酶(oxidase)/活性氧(ROS)/细胞外信号调节激酶(ERK)信号通路的影响。方法:将40只雄性SD大鼠分为正常组、模型组、阳性药组和山茱萸组,每组10只。除正常组外,其余各组按0.25 g/(kg·d)的剂量灌胃腺嘌呤溶液,连续给予21 d制备CRF模型。模型制备成功后,阳性药组按0.75 g/(kg·d)的剂量给予黄葵胶囊,山茱萸组按0.60 g/(kg·d)的剂量给予山茱萸提取物,正常组和模型组给予等量生理盐水,持续28 d。对肾组织进行病理学检查及评分,同时检测肾功能指标、血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量及肾脏中相关蛋白的含量。结果:正常组大鼠肾脏病理未观察到损伤迹象;模型组出现弥漫肾小球和肾小管萎缩、炎性细胞浸润和间质纤维化等病变;阳性药组肾小球和肾小管萎缩、炎性细胞浸润和间质纤维化等病变较模型组减轻;山茱萸组肾小球和肾小管萎缩、炎性细胞浸润和间质纤维化等病变程度介于模型组和阳性药组之间。与正常组比较,模型组炎症评分、纤维化评分及尿素氮(BUN)、肌酐(SCr)、尿蛋白(UP)、TNF-α、IL-6、α-平滑肌肌动蛋白(α-SMA)、NADPH氧化酶1(NOX1)、ROS、磷酸化ERK(p-ERK)含量均增加(P<0.05);与模型组比较,阳性药组和山茱萸组炎症评分、纤维化评分及BUN、SCr、UP、TNF-α、IL-6、α-SMA、NOX1、ROS、p-ERK含量均降低(P<0.05);与阳性药组比较,山茱萸组炎症评分、纤维化评分及BUN、SCr、UP、TNF-α、IL-6、α-SMA、NOX1、ROS、p-ERK含量均增加(P<0.05)。结论:山茱萸提取物可以通过抑制肾组织炎症反应和纤维化对CRF大鼠起到一定的保护作用,其机制可能与oxidase/ROS/ERK信号通路有关。
Objective:To discuss the effect of extract of cornus officinalis on renal protection and oxidase/reactive oxygen species(ROS)/extracellular signal-regulated kinase(ERK)signaling pathway in rats with chronic renal failure(CRF).Methods:A total of 40 male SD rats were divided into the normal group,the model group,the positive medicine group and the cornus officinalis group,10 rats in each group.Except for the normal group,the other groups were given gastric perfusion with adenine solution in 0.25 g/(kg·d).All groups were given continuous 21 days for preparing CRF model.After successful modeling,the positive medicine group was given 0.75 g/(kg·d)okra capsules;the cornus officinalis group was given extract of cornus officinalis of 0.60 g/(kg·d);the normal group and the model group were given normal saline of same volume.The medication was lasted for 28 days.The pathological examination of renal tissues was conducted,and renal tissues were scored;the indexes of the kidney function,and the contents of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in serum,and associated protein in the kidney were detected.Results:No signs of injury was observed in renal pathology in the normal group;there were lesions including renal glomerular and tubular diffuse atrophy,inflammatory cell infiltration and interstitial fibrosis in the model group;when compared with those in the model group,the three lesions in the positive medicine group were decreased;in the cornus officinalis group,the degree of the three lesions was between the model group and the positive medicine group.When compared with those in the normal group,the inflammation scores,the fibrosis scores,and the contents of blood urea nitrogen(BUN),Serum creatinine(SCr),urine protein(UP),TNF-α,IL-6,α-smooth muscle actin(α-SMA),NADPH oxidase1(NOX1),ROS and phosphorylation-ERK(p-ERK)in the model group were increased(P<0.05);when compared with those in the model group,the two scores and the nine contents in the positive medicine group and the cornus officinalis group were decreased(P<0.05);when compared with those in the positive medicine group,the two scores and the nine contents in the cornus officinalis group were increased(P<0.05).Conclusion:The extract of cornus officinalis has significant protective effect on CRF rats through inhibiting inflammatory response and fibrosis in renal tissues,whose mechanism is probably related to oxidase/ROS/ERK signaling pathway.
作者
丁国明
戢晴
韩颖敏
DING Guoming;JI Qing;HAN Yingmin
出处
《新中医》
CAS
2020年第18期14-18,共5页
New Chinese Medicine