不同产地木豆叶中5种黄酮类成分含量的比较及聚类分析

Comparison and Cluster Analysis of Five Flavonoids in Cajanus cajan Leaves from Different Producing Areas

[目的]建立木豆叶中5种黄酮类成分含量的测定方法,比较不同产地木豆叶中5种黄酮类成分的含量并对其进行聚类分析。[方法]采用HPLC-DAD建立木豆叶中5种黄酮类成分(荭草苷、牡荆苷、木犀草素、染料木素、球松素)的含量测定方法,色谱柱为Agilent Zobax Eclipse XDB-C 18(4.6 mm×250 mm,5μm),以甲醇(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱,流速为1.0 mL/min,检测波长330 nm,柱温为30℃;通过IBM SPSS 22.0对不同产地木豆叶进行聚类分析。[结果]5种对照品在相应浓度范围内与峰面积均呈现良好的线性关系;回收率均在90%~110%;不同产地木豆叶中5种黄酮类成分含量差异较大,基于5种黄酮类成分含量对不同产地的木豆叶聚类结果表明,木豆叶可分为2组,其中一组5种黄酮总量基本都大于0.5%,另一组则基本小于0.5%,但是并无明显产地聚类特性。[结论]该研究建立的木豆叶5种黄酮类物质含量测定方法准确、可靠,可用于木豆叶中5种黄酮成分的含量测定;根据聚类分析表明,不同产地木豆叶质量参差不齐,可以优选黄酮含量高的品种进行选育,从而保证药材来源的质量稳定性以及用药安全性。

[Objective]To establish a method for determining the content of five flavonoids in Cajanus cajan leaves and comparing the content of five flavonoids in Cajanus cajan leaves from different regions and perform cluster analysis.[Method]An HPLC method was developed for the determination of five flavones(Humulusside,vitexin,luteolin,genistein,pinostrobin)in the Cajanus cajan leaves.The chromatographic column was Agilent Zobax Eclipse XDB-C18(4.6 mm×250 mm,5μm);gradient elution using methanol(A)-0.1%formic acid aqueous solution(B)as the mobile phase;flow rate was set at 1.0 mL/min;detection wavelength was set 330 nm;column temperature was set at 30℃;the content of five flavonoids of Cajanus cajan leaves from different areas were detected using the above methods;cluster analysis of Cajanus cajan leaves of different origins by IBM SPSS 22.0 was conducted.[Result]The five reference products showed a good linear relationship with the peak area in the corresponding concentration range;the recovery rates were between 90%and 110%;the content of the five flavonoids in the leaves of Cajanus cajan leaves from different places varied widely.The clustering results based on the content of five flavonoids showed that Cajanus cajan leaves from different places can be divided into two groups.The total amount of flavonoids in one group is basically greater than 0.5%,and the other group is basically less than 0.5%,but there is no obvious clustering characteristics of origin.[Conclusion]The method for determining the content of five flavonoids in Cajanus cajan leaves is accurate and reliable.It can be used to determine the content of five flavonoids in Cajanus cajan leaves.According to the cluster analysis,the quality of Cajanus cajan leaves varies from place to place,and varieties with high flavonoid content can be selected for breeding,so as to ensure the quality stability of the medicinal materials and the safety of the medication.