深圳地区人群乙型肝炎病毒携带者HLA-DRB1等位基因多态性及关联性分析

Analysis of Allelic Polymorphism and Association of HLA-DRB1 in Hepatitis B Virus Carriers in Shenzhen

目的研究深圳地区人群乙型肝炎病毒携带者人类白细胞抗原(human leukocyte antigen,HLA)DRB1的等位基因多态性分布特征,探讨乙型肝炎病毒携带与HLA-DRB1等位基因之间的关联性。方法采用聚合酶链反应-直接测序分型技术(PCR-SBT)对163例深圳地区乙型肝炎病毒携带者(携带组)和314例健康人群(对照组)进行HLADRB1等位基因的检测,运用统计学方法比对分析两组人群的基因分型结果。结果对照组检出的HLA-DRB1等位基因有32种,其中占比大于5%的基因型有6种,分别为HLA-DRB1*09:01(16.40%),12:02(11.15%),15:01(9.87%),08:03(8.91%),11:01(6.05%)和04:05(5.10%);携带组中检出的HLA-DRB1等位基因有28种,其中占比大于5%的基因型有6种,分别为HLA-DRB1*09:01(20.55%),12:02(14.72%),15:01(9.20%),03:01(7.06%)、08:03(5.52%)和11:01(5.21%)。其中,携带组中HLA-DRB1*03:01检出率高于对照组,差异有统计学意义(P=0.010)。结论深圳地区人群感染乙肝病毒的机会与HLA-DRB1*03:01呈正相关。

Objective To study the distribution characteristics of allelic polymorphism of human leukocyte antigen(HLA)DRB1 in Hepatitis B virus carriers in Shenzhen area,and explore the association between Hepatitis B virus and HLA-DRB1 allele.Methods HLA-DRB1 alleles were detected in 163 Hepatitis B virus carriers(the carrying group)and 314 healthy people(the control group)in Shenzhen by polymerase chain react-direct sequencing typing(PCR-SBT).The genotyping results of the two groups were compared using statistical methods.Results There were 32 HLA-DRB1 alleles detected in the control group,among which 6 genotypes with a proportion greater than 5%were HLA-DRB1*09:01(16.40%),12:02(11.15%),15:01(9.87%),08:03(8.91%),11:01(6.05%)and 04:05(5.10%),respectively.And there were 28 alleles of HLA-DRB1 detected in the carrying group,including 6 genotypes with a proportion greater than 5%,which were HLA-DRB1*09:01(20.55%),12:02(14.72%),15:01(9.20%),03:01(7.06%),08:03(5.52%)and 11:01(5.21%),respectively.Among them,the detection rate of HLA-DRB1*03:01 in the carrying group was higher than that in the control group,and the P value showed a significant difference(P=0.010).Conclusion The chance of Hepatitis B virus infection in Shenzhen area was positively correlated with HLA-DRB1*03:01.