广西壮族自治区人群华支睾吸虫感染与MN血型基因的相关性研究

Correlation between Clonorchis Sinensis Infection and MN Blood Type Gene in Guangxi Zhuang Autonomous Region

目的调查广西壮族自治区人群华支睾吸虫高发人群与低感染人群的分布状态及MN血型基因的相关性,深入了解华支睾吸虫的感染情况,为预防与治疗华支睾吸虫病打下基础。方法以广西壮族自治区的武鸣区、贵港平南区两个代表性地区作为调查研究对象,以酶联免疫法随机筛查贵港平南区500例、武鸣区700例的15~65岁居民,分别采集3ml抗凝血与3ml不抗凝血,共检测1200例人群血清中的华支睾吸虫IgG抗体;通过血型血清学检测样本的红细胞血型糖蛋白MN血型,用基因分型的方法鉴定M^G与M^T的型别。结合两个区的调查总人数、华支睾吸虫抗体阳性率、阴性率、MN血型的血清学与基因分型作为统计数据。结果两个具有相同生活饮食习惯地区人群的华支睾吸虫感染率具有显著差异,即武鸣区华支睾吸虫感染率高达62%,其M基因频率、N基因频率、M^G基因频率和M^T基因频率分别为72%,28%,68.6%和31.4%。贵港平南区华支睾吸虫阳性感染率只有3.4%,其M基因频率、N基因频率、M^G基因频率和M^T基因频率分别为69%,31%,82.3%和17.7%。结论广西壮族自治区华支睾吸虫的感染率具有地区间的明显差异,而红细胞糖蛋白血型的M,N,M^G和M^T基因频率未发现明显差异,另外未发现饮食习惯与两区华支睾吸虫的易感因素直接相关。

Objective To investigate the distribution of Clonorchis sinensis in high incidence and low infection population in Guangxi Zhuang Autonomous Region and the correlation of MN blood group gene,so as to understand the infection situation of Clonorchis sinensis,and lay a foundation for the prevention and treatment of Clonorchis sinensis.Methods Two representative regions that Wuming and Guigang(Pingnan)District for the Guangxi Zhuang Autonomous Region were taken as the research objects.There were 1200 residents aged 15~65 years old that 500 cases in Pingnan District of Guigang and 700 cases in Wuming.The anticoagulant and nonanticoagulant blood for 3ml were collected and IgG antibodies of Clonorchis sinensis were detected by enzyme linked immunoassay(ELISA).The MN blood type was detected and the M^G and M^T types were identified by genotyping.Combined with the total number of people investigated in the two areas with the positive rate,negative rate of antibodies against Clonorchis sinensis and the serological and genotyping of MN blood type were taken as the statistical data.Results The infection rate of Clonorchis sinensis was significantly differentin two regions with the same living and eating habits.The infection rate of Clonorchis sinensis in Wuming area was as high as 62%,and the M gene frequency,the N gene frequency,the M^G gene frequency frequency and the M^T gene frequency frequency were 72%,28%,68.6%and 31.4%,respectively.But there’s a difference in Pingnan District of Guigang were the infection rate of Clonorchis sinensis was 3.4%,and the M gene frequency,the N gene frequency gene,the M^G gene frequency and the M^T gene frequency were 69%,31%,82.3%and 17.7%,respectively.Conclusion The infection rate of Clonorchis sinensis in two populations with the same living and eating habits was significantly different.But no significant difference was found about erythrocyte glycoprotein blood group M,N,M^G and M^T gene frequency.In addition,there was no direct correlation between dietary habits and susceptibility factors of Clonorchis sinensis.