2型糖尿病患者锰超氧化物歧化酶Ala-9Val基因多态性与颈动脉粥样硬化的相关性研究

Association between Manganese Superoxide Dismutase Gene Ala-9Val Polymorphism and Carotid Atherosclerosis in Patients with Type 2 Diabetes Mellitus

目的探讨2型糖尿病(type 2 diabetes mellitus,T2DM)患者锰超氧化物歧化酶(manganese-superoxide dismutase,Mn-SOD)Ala-9Val基因多态性与颈动脉粥样硬化(carotid atherosclerosis,CAS)的关系。方法选取九八七医院2017年1月~2019年1月的310例T2DM患者为T2DM组,根据颈动脉超声检查结果分为无CAS亚组和CAS亚组。另随机选取同期160例健康体检的志愿者作为对照组,采用聚合酶链反应-限制性片段长度多态性法(polymerase chain reaction restriction fragment length polymorphism,PCR-RFLP)检测Mn-SOD基因Ala-9Val位点基因型。分别比较各组间Ala-9Val位点基因型和等位基因分布频率。采用Logistic多因素回归分析法探究T2DM患者发生CAS的独立危险因素。结果对照组和T2DM组的Ala-9Val位点基因型分布频率差异无统计学意义(χ^2=4.210,P=0.122),两组的等位基因分布频率差异具有统计学意义(χ^2=4.487,P=0.034)。CAS亚组与无CAS亚组的Ala-9Val位点基因型和等位基因分布频率差异均具有统计学意义(χ^2=12.283,P=0.002;χ^2=10.984,P=0.001)。Logistics多因素回归分析结果显示,Val/Val基因型是T2DM患者发生CAS的独立危险因素(OR=1.865,95%CI 1.086~3.200,P=0.007)。结论T2DM患者Mn-SOD基因Ala-9Val位点多态性与CAS存在一定程度的相关性。

Objective To investigate the relationship between the Ala-9Val gene polymorphism of manganese superoxide dismutase(Manganese-Superoxide Dismutase,Mn-SOD)and carotid atherosclerosis(CAS)in patients with type 2 diabetes mellitus(T2DM).Methods A total of 310 cases of T2DM patients in No.987 Hospital from January 2017 to January 2019 were assigned to the T2DM group.According to the results of carotid ultrasound,the subjects were divided into no CAS subgroup and CAS subgroup.Besides,160 healthy volunteers during the same period were randomly assigned to the control group.PCR RFLP was used to detect the Ala-9 Val locus genotype of MN-SOD gene.Allele frequency and genotype of Ala-9Val locus were compared among groups.Logistic multivariate regression analysis was used to explore the independent risk factors for CAS in T2DM patients.Results There was no significant difference in Ala-9Val genotype distribution frequency between the control group and the T2DM group(χ^2=4.210,P=0.122),and the allele frequency difference between the two groups was statistically significant(χ^2=4.487,P=0.034).There were statistically significant differences in Ala-9Val genotype and allele distribution frequency between the CAS subgroup and the non-CAS subgroup(χ^2=12.283,P=0.002;χ^2=10.984,P=0.001).Logistic multifactor regression analysis results showed that Val/Val genotype was an independent risk factor for CAS in T2DM patients(OR=1.865,95%CI 1.086~3.200,P=0.007).Conclusion There was a certain degree of correlation between the polymorphism of Mn-SOD gene ALa-9Val and CAS in T2DM patients.