肺癌患者血浆miR-3151表达水平与临床病理特征的相关性研究

Study on the Correlation between the Expression of miR-3151 in Plasma and Clinicopathological Features in Patients with Lung Cancer

目的探讨肺癌患者血浆中miR-3151的表达及其与患者临床病理特征的关系。方法收集十堰市太和医院2018年10月~2019年10月确诊的120例肺癌患者(肺癌组)及同期120例体检健康者(对照组)的血浆样本,实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)技术检测两组血浆miR-3151的表达水平,分析血浆miR-3151的表达与肺癌患者临床病理特征的关系。受试者工作特征曲线(receiver operating characteristic curve,ROC)用以评估血浆miR-3151单独及联合细胞角蛋白19片段(cytokeratin-19 fragment,CYFRA21-1)、神经元特异性烯醇化酶(neuronspecific enolase,NSE)和癌胚抗原(carcinoembryonic antigen,CEA)对肺癌的诊断价值。结果肺癌组血浆miR-3151(nmol/L)显著高于对照组[86.356(19.893,305.155)vs 8.871(4.620,14.037)],差异有统计学意义(Z=-10.039,P<0.001)。血浆miR-3151的表达上调与肺癌患者淋巴结转移(Z=-2.336,P=0.019)和临床分期(Z=-2.628,P=0.009)相关。血浆miR-3151,CYFRA21-1,NSE及CEA单独用于诊断肺癌的曲线下面积(area under the cure,AUC)分别为0.875(95%CI:0.830~0.919),0.704(95%CI:0.639~0.770),0.769(95%CI:0.707~0.830)和0.632(95%CI:0.562~0.702),当miR-3151联合NSE时,诊断肺癌的AUC增加至0.909(95%CI:0.872~0.946),较单独诊断时有明显提高。结论miR-3151在肺癌患者血浆中表达上调,与肿瘤进展相关,有成为肺癌诊断标志物的潜能。

Objective To explore the expression level of plasma miR-3151 in lung cancer and its relationship with clinicopathological features.Methods The plasma samples of 120 lung cancer patients(lung cancer group)and 120 healthy subjects(control group)were collected from Taihe Hospital of Shiyan City from October 2018 to October 2019.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression level of plasma miR-3151 in the two groups.The clinical data of lung cancer patients were analyzed to explore the correlation between plasma miR-3151 and clinicopathological features.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of plasma miR-3151,cytokeratin 19 fragment(CYFRA21-1),neuron specific enolase(NSE)and carcinoembryonic antigen(CEA)for lung cancer.Results The expression level of plasma miR-3151(nmol/L)in lung cancer group was obviously higher than that in control group[86.356(19.893,305.155)vs 8.871(4.620,14.037)],and the difference was statistically significant(Z=-10.039,P<0.001).The upregulation of plasma miR-3151 showed association with the lymph node metastasis(Z=-2.336,P=0.019)and clinical stage(Z=-2.628,P=0.009)of patients with lung cancer.The area under the curve(AUC)of plasma miR-3151,CYFRA21-1,NSE and CEA for the diagnosis of lung cancer were 0.875(95%CI:0.830~0.919),0.704(95%CI:0.639~0.770),0.769(95%CI:0.707~0.830)and 0.632(95%CI:0.562~0.702),respectively.When miR-3151 combined with NSE,the AUC for diagnosis of lung cancer increased to 0.909(95%CI:0.872~0.946),which was significantly higher than that of diagnosis alone.Conclusion The expression of plasma miR-3151 in lung cancer was up-regulated,which was related to tumor progression,and it has the potential to become a diagnostic marker for lung cancer.