多穗金粟兰的化学成分及其抗炎活性研究

Chemical constituents from Chloranthus multistachys and their anti-inflammatory activity

对多穗金粟兰(Chloranthus multistachys Pei)全草的化学成分及其抗炎活性进行研究。利用硅胶柱色谱等多种色谱方法进行分离纯化和波谱学方法进行结构鉴定,从多穗金粟兰中分离了22个化合物,分别鉴定为:(7S,8R)-dihydrodehydrodiconiferyl alcohol(1)、7-ketositosterol(2)、6β-羟基豆甾-4-烯-3-酮(3)、6α-羟基豆甾-4-烯-3-酮(4)、4β,8β-二羟基-5α(H)-桉叶-7(11)-烯-8,12-内酯(5)、13-epitorulosol(6)、15-nor-14-oxolabda-8(17),12E-dien-19-oic acid(7)、4α-hydroxy-8,12-epoxyeudesma-7,11-diene-1,6-dione(8)、金粟兰素A(9)、fortunilide K(10)、(1E,4Z)-8-hydroxy-6-oxogermacra-1(10),4,7(11)-trieno-12,8-1actone(11)、henrilabdane B(12)、2′-羟基-4,3′,4′,6′-四甲氧基查尔酮(13)、curcolonol(14)、zederone epoxide(15)、银线草醇B(16)、环银线草醇A(17)、henrilabdane A(18)、12-(3-methyl-furan)-labd-8(17)-en-19-oic acid(19)、ent-8(9)-pimarene-20-hydroxy-16-nor-15-oic acid(20)、反式-N-阿魏酰酪胺(21)、grossamide(22)。其中化合物化合物2、4、21和22为首次从金粟兰属中分离得到,化合物1~10、18~22为首次从多穗金粟兰中分离得到。此外,采用脂多糖(LPS)诱导的RAW264.7细胞对化合物5~17进行了抗炎活性筛选。结果表明化合物5、10、11和13有较好的抗炎活性,其一氧化氮(NO)抑制率分别为41.22%±8.28%、40.32%±15.14%、58.79%±8.16%、43.85%±10.04%。

Chloranthus multistachys Pei is a perennial herb and has been used as folk medicine to treat bone fracture,hemostasis swelling pain and pruritus cutanea for centuries in China.In this paper,the chemical constituents of C.multistachys and their anti-inflammatory activities were investigated.Twenty-two compounds were isolated by a variety of chromatographic techniques and identified as(7S,8R)-dihydrodehydrodiconiferyl alcohol(1),7-ketositosterol(2),6β-hydroxystigmast-4-en-3-one(3),6α-hydroxystigmast-4-en-3-one(4),4β,8β-dihydroxy-5α(H)-eudesm-7(11)-en-8,12-olide(5),13-epitorulosol(6),15-nor-14-oxolabda-8(17),12E-dien-19-oic acid(7),4α-hydroxy-8,12-epoxyeudesma-7,11-diene-1,6-dione(8),spicachlorantin A(9),fortunilide K(10),(1E,4Z)-8-hydroxy-6-oxogermacra-1(10),4,7(11)-trieno-12,8-1actone(11),henrilabdane B(12),2′-hydroxy-4,3′,4′,6′-tetramethoxychalcone(13),curcolonol(14),zederone epoxide(15),shizukaol B(16),cycloshizukaol A(17),henrilabdane A(18),12-(3-methyl-furan)-labd-8(17)-en-19-oic acid(19),ent-8(9)-pimarene-20-hydroxy-16-nor-15-oic acid(20),N-trans-feruloyltyramine(21)and grossamide(22).Compounds 2,4,21 and 22 were isolated from the genus Chloranthus for the first time,compounds 1-10 and 18-22 were isolated from C.multistachys for the first time.Additionally,compounds 5-17 were screened for anti-inflammatory activity by LPS-induced RAW264.7 cells.The results showed that compounds 5,10,11 and 13 exhibited moderate anti-inflammatory activity with the NO inhibition rates of 41.22%±8.28%,40.32%±15.14%,58.79%±8.16%and 43.85%±10.04%,respectively.