荧光定量聚合酶链式反应在羊水快速产前诊断中的应用

Application of QF-PCR in amniotic fluid rapid prenatal diagnosis

目的 探讨荧光定量聚合酶链式反应(QF-PCR)在快速产前诊断中的临床应用价值.方法 应用QF-PCR技术对2018年5月7日至2019年5月7日于扬州市妇幼保健院产前诊断中心行产前诊断的1 190例产前羊水样本进行13、18、21、X及Y的非整倍体产前诊断并与核型分析结果对比.结果 1 190例羊水QF-PCR共诊断出33例各种染色体异常,包括21-三体20例;18-三体4例及性染色体异常9例.结论 作为常见的快速诊断方法,QF-PCR诊断结果快速、准确,但还不能完全代替传统的核型分析.

Objective To assess the clinical value of quantitative fluorescent polymerase chain reaction(QF-PCR)in rapid prenatal diagnosis.Methods From May 2018 to May 2019,1190 amniotic fluid samples were dected by QF-PCR to check out aneuploidies of 13,18,21,X and Y,then the results were compared with those of traditional karyotype analysis.Results The 33 abnormities including 20 cases with trisomy 21,4 cases with trisomy 18 and 9 abnormities of sex chromosomes were checked out in 1190 amniotic fluid samples by rapid diagnosis.Conclusion As a common rapid diagnostic method,QF-PCR has advantages of rapid and accurate,but still cannot completely replace the conventional karyotype analysis.