摘要
CRISPR/Cas system,originally developed as genetic editing tool,also shows great potentials for nucleotide detection.A recent study published in Molecular Cell(Freije et al.,2019)developed a Cas13a-based CARVER(Cas13-assisted restriction of viral expression and readout)to detect RNA viruses such as lymphocytic choriomeningitis,influenza A and vesicular stomatitis,which provided a potential expanded application for the detection of a broad range of viral nucleotides in disease diagnosis.
基金
The work was supported by grants from the National Key Research and Development Program of China(Grant No.2017YFA0103902)
the National Natural Science Foundation of China(Grant No.31771283)
the Fundamental Research Funds for the Central Universities of Tongji University(Grant No.22120190210)
the SAAS Program for Excellent Research Team(Grant No.2017-B-07)
the Shanghai Leading Talent Project(Grant No.2017067)
the Shanghai Fresh Corn Technology System Project(Grant No.2017-10).
