苦参碱对大鼠肝缺血再灌注损伤后肝功能及MAPK通路的影响

Influence of matrine on liver function and MAPK signaling pathway after hepatic ischemic reperfusion injury in rats

目的探讨苦参碱(matrine,MT)对大鼠肝缺血再灌注损伤后肝功能及MAPK通路的影响。方法大鼠随机分为假手术(Sham)组、苦参碱(MT)组和肝缺血再灌注损伤模型(I/R)组。MT组腹腔注射MT(20、40 mg/kg),Sham和I/R组分别注入等体积生理盐水,连续7 d。除Sham组外,I/R和MT组大鼠建立肝缺血再灌注模型,观察凋亡和病理形态学改变,检测各组血清转氨酶、肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)浓度及丝裂原活化蛋白激酶(MAPK)通路ERK1/2、p38和JNK蛋白表达。结果与I/R组比较,MT组显著降低I/R术后转氨酶、MDA并增加SOD含量,显著降低TUNEL凋亡细胞数量,抑制MKK7、p-JNK和p-p38蛋白表达。结论MT对大鼠肝缺血再灌注损伤有保护作用,机制与抑制MKK7/JNK、p38磷酸化、抗凋亡有关。

Objective To investigate the effect of matrine(MT)on hepatic ischemic/reperfusion(I/R)injury and MAPK pathway.Methods Rats were randomized into the sham group,the vehicle-treated hepatic I/R group and two matrine-treated(20,40 mg/kg)groups for consecutive 7 days.The hepatic I/R and MT-treated groups were subjected to I/R model.The animals were then sacrificed to collect the serum and the left liver lobe for assay.Results Hepatic function was protected,as evidenced by significantly reduced alanine aminotransferase(ALT),aspartate amino transferase(AST)and malondialdehyde(MDA)levels,and increased superoxide dismutase(SOD)in the MT-treated group as compared with the I/R group.Meantime,TUNEL positive cells were significantly decreased in the MT-treated animals.MT also inhibited the expression of MKK7,p-JNK and p-p38.Conclusion MT attenuates hepatic I/R injury in rats,which is related to inhibition of MKK7/JNK,p38 MAPK phosphorylation and cell apoptosis.