植物Dof基因结构特点及功能研究进展

Advances on the Structural Characteristics and Function of Dof Gene in Plant

DNA结合单锌指(DNA binding with one zinc finger,Dof)蛋白是由多基因共同编码的只在植物中存在的一类转录因子,其N-末端含有高度保守的由52个氨基酸残基组成的C2-C2型单锌指结构域,可以与DNA和蛋白质结合并相互作用;C-末端是特异转录调控结构域,因其序列具有多变性,导致Dof基因功能的多样性。随着基因组学和生物信息学的发展,近年来已有多个物种的Dof蛋白相继被报道出来。Dof基因主要参与植物的生长发育、碳氮代谢、非生物胁迫及开花调控等生物学过程。综述了植物Dof转录因子的结构特性、已经报道的Dof基因数目及生物学功能,在此基础上讨论了毛竹开花研究存在的问题,并提出未来的展望,以期为进一步研究Dof转录因子提供参考。

DNA binding with one zinc finger(Dof)is a kind of specific transcription factors only in plant,which are encoded by multiple genes.The N-terminus contains a highly-conserved C2-C2 single zinc finger domain consisting of 52 amino acid residues,which could bind and interact with DNA and proteins.The C-terminus is a specific transcriptional regulatory domain,its variable amino acid sequence results in the functional diversity of Dof genes.With the development of genomics and bioinformatics,Dof proteins of multiple species have been reported in recent years.The Dof genes are mainly involved in biological processes,such as plant growth and development,carbon and nitrogen metabolism,abiotic stress,and flowering regulation.Hereby we review the structural characteristics of Dof transcription factors,the number of reported Dof genes and their biological functions.We also discuss the current issues and the future prospects in studying moso bamboo flowering,aiming to provide a reference for the further study of Dof transcription factors.