草鱼仔鱼耳石茜素络合物标记特征研究

The Marking Characteristics of Otoliths of Larval Grass Carp(Ctenopharyngodon idellus)by Immersion in Alizarin Complexone

草鱼(Ctenopharyngodon idellus)是我国主要增殖放流经济鱼种之一,探索有效的标志方法对其增殖放流的技术保障和效果评价无疑至关重要。本研究对草鱼仔鱼耳石进行了1 d茜素络合物(ALC)荧光标记,以确认该方法的可行性及适宜条件。结果显示,微耳石、矢耳石和星耳石均能被标记。标记环在可见光及荧光下均能被观察到。其中,后者使用蓝色激发光(WBS)观察效果最佳。总体上,微耳石的标记效果最好,矢耳石次之。ALC浸泡对鱼体的生存及生长无显著影响,耳石上荧光信号出现和消失与鱼体开始浸泡和结束均存在约12h的时滞。浸泡标记过的实验鱼在经过长达60 d的恢复续养后,标记环仍清晰可见,表明ALC标记效果明显且可长期存在。该方法对草鱼仔鱼的增殖放流标记具有较强的可行性。

The grass carp Ctenopharyngodon idellus is one of the main economic fish species for release and resource enhancement in China.In this study,the otoliths of larval grass carps were marked for 1 d by alizarin complexone(ALC)to confirm the feasibility and appropriate conditions for the method.The results showed that lapillus,sagitta,and asteriscus could be successfully marked.The marking rings were observed under both visible light and fluorescence,the latter of which was most obviously observed using blue excitation light.Overall,lapillus had the best marking effect,followed by sagitta.ALC immersion had no significant effect on fish survival and growth.The appearance and disappearance of fluorescent signals on the otolith,the beginning and end of immersion of the fish had a time lag of about 12 h.At 60 d of recovery of the culture after immersion,the ALC mark ring was still visible,indicating that the ALC-marked effect was evident and could exist for quite a long time.This method has a strong feasibility for marking and effect assessment of the larval grass carp for future resource enhancement and release.