多囊卵巢综合征患者血清miR-155、PDCD4表达及其临床意义

Expressions and clinical significance of miR-155 and PDCD4 levels of women with polycystic ovary syndrome

目的:检测多囊卵巢综合征(PCOS)患者血清中微小RNA-155(miR-155)及程序性细胞死亡因子(PDCD4)的表达情况,探讨其临床意义。方法:收集2015年10月-2019年12月在本院确诊的19~25岁PCOS患者128例(PCOS组)及年龄相匹配体检健康女性60例(对照组)。荧光实时定量PCR法测定两组对象血清miR-155水平,免疫吸附法检测血清中PDCD4蛋白表达;Pearson法分析血清miR-155及PDCD4表达与PCOS患者肥胖、脂质异常及胰岛素抵抗等指标的相关性。结果:血清miR-155、PDCD4蛋白水平,PCOS组均高于对照组,PCOS组中肥胖患者高于非肥胖患者,脂质异常患者高于非脂质异常患者,胰岛素抵抗患者高于非胰岛素抵抗患者(P均<0.05)。血清miR-155水平与PCOS患者体质指数、总胆固醇含量、胰岛素抵抗指数呈正相关(r=0.37、0.47、0.45,P均<0.05),血清PDCD4蛋白水平与PCOS患者体质指数、总胆固醇含量、胰岛素抵抗指数正相关(r=0.39、0.46、0.43,P均<0.05)。结论:PCOS患者血清miR-155及PDCD4高表达,且与PCOS患者肥胖、脂质异常及胰岛素抵抗有关。

Objective: To detect the expressions of serum microRNA-155(miR-155) and programmed cell death factor(PDCD4) of women with polycystic ovary syndrome(PCOS), and to explore its clinical significance. Methods: 128 women with PCOS in 19-25 years old were selected in study group and 60 healthy women in 19-25 years old were selected in control group from October 2015 to December 2019. The level of serum miR-155 of women in the two groups was determined by real-time quantitative PCR(qRT-PCR), and the expression of PDCD4 protein was detected by ELISA. Pearson method was used to analyze the correlations between the expressions of serum miR-155 and PDCD4 of women with PCOS and their indexes of obesity, lipid abnormality, and insulin resistance. Results: The levels of serum miR-155 and PDCD4 protein of women in the study group were significant higher than those of women in the control group(P<0.05). In the study group, the levels of serum miR-155 and PDCD4 protein of women with insulin resistance were significant higher than those of women without insulin resistance, the levels of serum miR-155 and PDCD4 protein of women with obesity were significant higher than those of women without obesity, the levels of serum miR-155 and PDCD4 protein of women with abnormal lipid level were significant higher than those of women with normal lipid level, the levels of serum miR-155 and PDCD4 protein of women with insulin resistance were significant higher than those of women without insulin resistance(all P<0.05). The levels of serum miR-155 was positively correlated with body mass index, total cholesterol level, and insulin resistance index of women with PCOS(r=0.37, 0.47, 0.45, P<0.05). And the level of serum PDCD4 protein was positively correlated with body mass index, total cholesterol level, insulin resistance index of women with PCOS(r=0.39, 0.46, 0.43, P<0.05). Conclusion: The expressions of serum miR-155 and PDCD4 of women with PCOS are high,which are related to obesity,lipid abnormality,and insulin resistance of women with PCOS.