TLR3基因多态性与复发性流产相关性分析

Correlation between TLR3 gene polymorphism of women and the rate of recurrent abortion

目的:探讨Toll样受体3(TLR3)基因单核苷酸多态性与复发性流产(RSA)的关系。方法:选择2015年1月-2019年1月本院妇产科收治的305例RSA患者(RSA组)和同期200例正常妊娠孕妇(对照组),均采用聚合酶链反应-限制性内切酶片断长度多态性(PCR-RFLP)法检测外周血中rs5743305、rs 3775290位点TLR3基因多态性,观察组间基因型分布差异,分析rs5743305、rs 3775290位点TLR3基因型与RSA易感和ACA抗体相关性。结果:RSA组rs5743305位点以AA基因型(54.1%)、A等位基因(66.4%)为主,rs 3775290位点以CC基因型(44.9%)、C等位基因(58.4%)为主;rs5743305位点AA基因型、rs 3775290位点以CC基因型ACA-IgM(15.24±6.19)、ACA-IgG(15.34±5.98)水平高于其它基因型(P<0.05)。logistic回归分析,rs5743305位点携带AA基因型、rs 3775290位点携带CC基因型孕妇发生RSA风险较高(P<0.05)。结论:rs5743305位点携带AA基因型、rs 3775290位点携带CC基因型孕妇是RSA易发生人群。

Objective: To investigate the relationship between single nucleotide polymorphisms of Toll-like receptor 3(TLR3) gene of women and the rate of recurrent spontaneous abortion(RSA). Methods: 305 women with RSA were selected in study group, and 200 normal pregnant women were selected in control group from January 2015 to January 2019. The polymerase chain reaction-restriction enzyme fragment length polymorphism(PCR-RFLP) was used for detecting the genes polymorphism on rs 5743305 and rs 3775290 TLR3 locus in peripheral blood of these women. The genotype distribution differences between the two groups were observed. The relationship between TLR3 genotypes of rs5743305 and rs 3775290 locus and RSA susceptibility or ACA antibodies(ACA-IgM, ACA-IgG) was analyzed. Results: In the study group, AA genotype(54.10%) and A allele(66.39%) of rs5743305 locus of women were the main, and CC genotype(44.92%) and C allele(58.36%) of rs 3775290 locus of women were the main. The levels of ACA-IgM and ACA-IgG of AA genotype on rs5743305 locus and CC genotype on rs 3775290 locus were 15.24±6.19 and 15.34±5.98, respectively, which were siginificant higher than those of other genotypes(P<0.05). Logistic regression analysis showed that women with rs5743305 locus carrying AA genotype and rs 3775290 locus carrying CC genotype had significant higher risk of RSA(P<0.05). Conclusion: The women with rs5743305 locus carrying AA genotype and rs 3775290 locus carrying CC genotype are included in RSA susceptible population.