IC-IPAD同时测定运动饮料中20种氨基酸和6种糖

Simultaneous determination of twenty amino acids and six sugars in sports drinks by IC-IPAD

建立了梯度淋洗-离子色谱-积分脉冲安培检测法同时测定运动饮料中20种氨基酸和6种糖的分析方法。通过对梯度淋洗条件、色谱柱温度、p H以及前处理条件等实验影响因素的考察,优化出适合同时检测26种组分的分析方法。结果表明,样品经调节pH沉淀蛋白后,再由RP净化柱处理,采用多级梯度淋洗模式,在35℃的色谱柱温度和样品溶液pH值为5.2~6.7条件下进行分离检测,26种组分在0.5~50.0 mg/L质量浓度范围内线性良好,相关系数(R2)为0.990~0.999,检出限为0.001~0.060 mg/L,定量限为0.003~0.200 mg/L。加标试验分别添加1 mg/L,10 mg/L和50 mg/L 3个水平,加标回收率达到86.2%~105.0%(n=5),相对标准偏差(RSD)为1.9%~5.3%。该方法高效、简便、灵敏、准确,可用于运动饮料中多种氨基酸和糖的同时测定分析。

A method of gradient elution ion chromatography with integral pulse amperometric detection for the simultaneous determination of 20 amino acids and 6 sugars in sports drinks was established.Through the investigation of the experimental factors such as gradient elution conditions,column temperature,pH and pretreatment conditions,the experimental method suitable for the simultaneous detection of 26 components was optimized.The results showed that the protein was precipitated by adjusting the pH of the sample,and then treated by RP purification column.The separation and detection were carried out under the conditions of column temperature 35℃and solution pH 5.2-6.7 by using multi-stage gradient elution mode.The linearity of 26 components was good in the concentration range of 0.5-50.0 mg/L,and the correlation coefficient R2 was 0.990-0.999.The detection limit was 0.001-0.060 mg/L,and the quantitative limit was 0.003-0.200 mg/L.Three levels of 1 mg/L,10 mg/L and 50 mg/L were added in the standard addition experiment,the recovery rates were 86.2%-105.0%(n=5),and relative standard deviation(RSD)was 1.9%-5.3%.The method was efficient,simple,sensitive and accurate,and could be used for the simultaneous determination of multi-amino acids and sugars in sports drinks.