含多种农药残留的茶叶自然污染基质标准样品的研制

Development of tea matrix reference material containing various pesticide residues

目的建立基于药物代谢的含多种农药残留的茶叶基质标准物质的研制与定值方法。方法在茶树种植过程中喷施农药,使药物在茶树中自然代谢,从而获得有农药残留的自然污染样本,比较了不同制茶工艺及辐照灭菌对农药残留的影响,经均质、真空包装及辐照处理,获得了一批含有多种农药残留的茶叶自然污染样本。结果经均匀性和稳定性检验,表明样本在最小取样量下的瓶间及瓶内均匀性良好,室温下可稳定保存至少60d。结论所研制的标准样品为自然污染的茶叶,与日常检测样品基质完全一致,可应用于相关检测方法的开发、验证和检测结果的质量控制等。

Objective To establish a method for preparing and certifying a kind of tea matrix reference material containing various pesticide residues based on the metabolism of pesticides in plants.Methods Various pesticides were applied in the process of tea planting to make the pesticides metabolize naturally in the tea trees,so as to obtain the natural contaminated samples with pesticide residues in tea.The effects of different tea making techniques and irradiation sterilization on pesticide residues were compared.After homogenization,vacuum packaging and irradiation treatment,a batch of tea natural contaminated samples containing various pesticide residues were obtained.Results The uniformity and stability tests showed that the samples were well uniform between and within bottles at the minimum test size and could be stored stably at room temperature for at least 60 days.Conclusion The developed tea matrix reference material is naturally contaminated tea,which is completely consistent with the daily test sample matrix,and can be applied to the development and verification of related test methods and the quality control of test results.