徐州市禽源弯曲菌的分离鉴定及耐药性分析

Isolation, identification and drug resistance analysis of Campylobacter from poultry in Xuzhou

目的解徐州市市售禽肉中弯曲菌的污染状况及对抗生素的耐药情况。方法随机采集农贸市场及超市新鲜或冷冻禽肉72份,采用滤膜法分离培养弯曲菌;采用琼脂稀释法测定分离出的菌株对6类11种抗生素的耐药性。结果 72份样品中共检出29份弯曲菌,总检出率为40.28%(29/72),空肠弯曲菌和结肠弯曲菌检出率分别为19.44%(14/72)和20.83%(15/72);新鲜禽肉和冷冻禽肉检出率分别为68.72%(22/32)和17.5%(7/40),差异有统计学意义(χ^2=19.412,P<0.05),空肠弯曲菌在新鲜禽肉和冷冻禽肉中检出率分别为34.38%(11/32)和7.5%(3/40)差异有统计学意义(χ^2=8.198,P<0.05),结肠弯曲菌在新鲜禽肉和冷冻禽肉中检出率分别为34.38%(11/32)和10%(4/40)差异有统计学意义(χ^2=6.404, P<0.05);空肠弯曲菌耐药较高的5种抗生素为萘啶酸100.00%(14/14)、环丙沙星100.00%(14/14)、四环素100.00%(14/14)、氟苯尼考64.29%(9/14),庆大霉素28.57%(4/12);结肠弯曲菌耐药较高的5种抗生素为为萘啶酸100.00%(15/15)、环丙沙星100.00%(15/15)、四环素100.00%(15/15)、链霉素86.67%(13/15),庆大霉素80%(12/15);2种弯曲菌的多重耐药性均为100.00%。结论徐州市市售禽肉中弯曲菌污染率较高,其中新鲜禽肉较冷冻禽肉中检出率高,且弯曲菌耐药情况较为严重。

Objective To investigate the contamination and antibiotic resistance of Campylobacter in poultry meat in Xuzhou city.methods Totally 72 samples of fresh or frozen poultry meat were randomly collected from farmers’markets and supermarkets,Campylobacter was isolated and cultured by filtration membrane method.The resistance of the isolated strains to 6 categories and 11 antibiotics were determined by the agar dilution method.Results A total of 29 cases of Campylobacter were detected in 72 samples,with a total detection rate of 40.28%(29/72).The detection rates of Campylobacter jejuni and Campylobacter colon were 19.44%(14/72)and 20.83%(15/72),respectively.The detection rate of fresh and frozen poultry meat were 68.72%(22/32)and 17.5%(7/40),respectively,with statistically significant difference(χ^2=19.412,P<0.05).The detection rate of Campylobacter jejuni in fresh poultry meat and frozen poultry meat was 34.38%(11/32)and 7.5%(3/40),the difference was statistically significant(χ^2=8.198,P<0.05).The detection rate of Campylobacter coli in fresh poultry meat and frozen poultry meat was 34.38%(11/32)and 10%(4/40),and the difference was statistically significant(χ^2=6.404,P<0.05).The 5 antibiotics with higher resistance to Campylobacter jejuni were nalidixic acid 100.00%(14/14),ciprofloxacin 100.00%(14/14),tetracycline 100.00%(14/14),and florfenicol 64.29%(9/14),gentamicin 28.57%(4/12).The 5 antibiotics with high resistance to Campylobacter coli were nalidixic acid 100.00%(15/15),ciprofloxacin 100.00%(15/15),tetracycline 100.00%(15/15),streptomycin 86.67%(13/15),gentamicin 80%(12/15).The multidrug resistance of both Campylobacter species was 100.00%.Conclusion The contamination rate of Campylobacter in commercial poultry meat sold in Xuzhou is relatively high.Among them,the detection rate of fresh poultry meat is higher than that in frozen poultry meat,and Campylobacter resistance is more serious.