QuEChERS-高效液相色谱-串联质谱法测定火锅调料中罂粟壳

Determination of poppy husk in hotpot seasoning by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

目的建立QuEChERS-高效液相色谱-串联质谱法测定火锅调料中的吗啡、可待因、罂粟碱、蒂巴因、那可丁5种生物碱含量的方法。方法样品通过QuEChERS法处理后,样品液采用C18色谱分离柱,以10mmol/L甲酸铵溶液(含0.1%甲酸)-乙腈(含0.1%甲酸)作为流动相进行梯度洗脱,电喷雾正离子多反应监测模式检测。结果吗啡、可待因的线性范围为5.0~250.0 ng/mL,相关系数为0.9990~0.9994,最低检出限为2.0 ng/mL,罂粟碱、那可丁、蒂巴因的线性范围为1.0~50.0 ng/mL,相关系数为0.9998~0.9999,最低检出限为0.1 ng/mL。平均回收率为84.5%~100.5%,相对标准偏差为0.8%~3.1%。结论该方法快速、简单、灵敏、准确,适用于火锅调料中罂粟壳的检测。

Objective To establish a method for the determination of morphine,codeine,papaverine,thebaine,narcotine in hotpot seasoning by QuEChERS-high performance liquid chromatography-tandem mass spectrometry.Methods The sample was processed by the QuEChERS method,then separated by C18 chromatographic column using 10 mmol/L ammonium formate solution(containing 0.1%formic acid)-acetonitrile(containing 0.1%formic acid)as the mobile phase for gradient elution,and detected by electrospray positive ion under multiple reaction monitoring mode detection.Results The linear ranges of morphine and codeine were 5.0-250.0 ng/mL,the correlation coefficients were 0.9990-0.9994,the minimum detection limit was 2.0 ng/mL,the linear ranges of papaverine,nacodine and theibain were 1.0-50.0 ng/mL,the correlation coefficients were 0.9998–0.9999,and the minimum detection limit was 0.1 ng/mL.The average recovery rates were 84.5%-100.5%,and the relative standard deviations were 0.8%–3.1%.Conclusion This method is rapid,simple,sensitive and accurate,and suitable for the detection of poppy shell in hotpot seasoning.