光皮木瓜化学成分研究及其神经保护活性

Chemical constituents from the fruits of Chaenomeles sinensis and their neuroprotective activity

目的研究光皮木瓜Chaenomeles sinensis(Thouin)Koehne的化学成分及其神经保护活性。方法光皮木瓜50%丙酮提取物采用Diaion HP-20、Toyopearl HW-40、MCI Gel CHP-20、ODS、硅胶、半制备液相进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法检测化合物对皮质酮诱导的大鼠嗜铬细胞瘤细胞(PC-12细胞)损伤的保护活性。结果从中分离得到9个化合物,分别鉴定为8-hydroxy-2,7-dimethyl-2E,4E-decadienedioic acid 1-β-D-glucopyranyl ester 10-methyl ester(1)、monomethyl glansreginate(2)、2,7-dimethyl-2,4-diene-deca-α,ω-diacid(3)、(6S)-menthiafolic acid(4)、(4E,6E)-2,7-dimethyl-8-hydroxyoctadienoic acid(5)、glansreginic acid(6)、2,7-dimethyl-2E,4E-octadienedioic acid(7)、9ξ-O-β-D-glucopyranosyloxy-5-megastigmen-4-one(8)、icariside B9(9)。在浓度为5μmol/L时,化合物3~5、7可显著提高皮质酮诱导的PC-12损伤的细胞活力。结论所有化合物均为首次从该植物中分离得到,且化合物3~5、7具有一定的神经保护活性。

AIM To study the chemical constituents from the fruits of Chaenomeles sinensis(Thouin)Koehne and their neuroprotective activity.METHODS The 50%acetone extract from C.sinensis was isolated and purified by Diaion HP-20,Toyopearl HW-40,MCI Gel CHP-20,ODS,silica gel and semi-preparative-HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their protective effect on rat adrenal pheochromocytoma(PC-12)injury induced by corticosterone through MTT method.RESULTS Nine compounds were isolated and identified as 8-hydroxy-2,7-dimethyl-2E,4E-decadienedioic acid 1-β-D-glucopyranyl ester 10-methyl ester(1),monomethyl glansreginate(2),2,7-dimethyl-2,4-diene-deca-α,ω-diacid(3),(6S)-menthiafolic acid(4),(4E,6E)-2,7-dimethyl-8-hydroxyoctadienoic acid(5),glansreginic acid(6),2,7-dimethyl-2E,4E-octadienedioic acid(7),9ξ-O-β-D-glucopyranosyloxy-5-megastigmen4-one(8),icariside B9(9).At a concentration of 5μmol/L,compounds 3-5,7 could significantly increase the cell viability of corticosterone-induced PC-12 damage.CONCLUSION All the compounds are isolated from this plant for the first time,and compounds 3-5,7 have certain neuroprotective activity.