AP-2α ITGB1在宫颈鳞癌中的表达及与预后的相关性分析

Expressions of AP-2αand ITGB1 in Cervical Squamous Cell Carcinoma and The Correlation with Prognosis

目的:通过检测转录因子激活蛋白-2α(AP-2α)及整合素β1蛋白(ITGB1)在宫颈鳞癌中表达水平,分析二者与宫颈鳞癌患者预后关系。方法:选取2013年10月至2016年12月本院收治的93例宫颈鳞癌患者,另选取同期因子宫肌瘤需行全子宫切除术患者93例作为对照。术中分别收集入组者宫颈鳞癌组织及正常宫颈组织,免疫组织化学染色法检测AP-2α、ITGB1蛋白表达水平,分析二者水平与宫颈鳞癌患者临床病理特征及预后关系,Cox回归模型分析影响宫颈鳞癌患者不良预后危险因素。结果:宫颈鳞癌组织中AP-2α蛋白阳性表达率显著低于正常宫颈组织,ITGB1蛋白阳性表达率显著高于正常宫颈组织(P<0.05)。AP-2α、ITGB1蛋白表达水平与肌层浸润、FIGO分期及是否有淋巴结转移有关(P均<0.05)。宫颈鳞癌组织中AP-2α与ITGB1蛋白表达水平呈负相关(r=-0.582,P<0.05)。AP-2α阳性组患者3年总生存率显著高于AP-2α阴性组患者,ITGB1阳性组患者3年总生存率显著低于ITGB1阴性组患者(P<0.05)。FIGO分期Ⅲ期、淋巴结转移、AP-2α阴性、ITGB1阳性是影响宫颈鳞癌患者不良预后独立危险因素(P均<0.05)。结论:与正常宫颈组织相比,宫颈鳞癌组织中AP-2α表达水平降低,ITGB1表达水平升高,二者与宫颈鳞癌肌层浸润、FIGO分期、淋巴结转移及患者生存率有关,是宫颈鳞癌患者不良预后独立危险因素,二者可能作为标志物用于预后评估。

Objective:To analyze the relationships between AP-2αand ITGB1 expressions and prognosis of cervical squamous cell carcinoma.Methods:93 cases of cervical squamous cell carcinoma were selected from October 2013 to December 2016 in our hospital,and 93 cases of hysteromyoma patients who needed total hysterectomy at the same time were selected as control.Cervical squamous cell carcinoma and normal cervical tissue were collected during operation,the expression levels of AP-2αand ITGB1 were detected by immunohistochemical staining,the relationship between the two levels and clinicopathological features,prognosis of cervical squamous cell carcinoma were analyzed,and Cox regression model was used to analyze the risk factors of adverse prognosis in patients with cervical squamous cell carcinoma.Results:The positive expression rate of AP-2αprotein in cervical squamous cell carcinoma was significantly lower than that in normal cervical tissue,and the positive expression rate of ITGB1 protein was significantly higher than that in normal cervical tissue(P<0.05).The expression levels of AP-2αand ITGB1 were correlated with myometrial invasion,FIGO stage and lymph node metastasis(P<0.05).There was a negative correlation between AP-2αand ITGB1 expressions in cervical squamous cell carcinoma(r=-0.582,P<0.05).The 3-year overall survival rate of AP-2αpositive group was significantly higher than that of AP-2αnegative group,and the 3-year overall survival rate of ITGB1 positive group was significantly lower than that of ITGB1 negative group(P<0.05).FIGO stage III,lymph node metastasis,AP-2αnegative and ITGB1 positive were independent risk factors for adverse prognosis of cervical squamous cell carcinoma(all P<0.05).Conclusion:Compared with normal cervical tissues,the expression of AP-2αin cervical squamous cell carcinoma is decreased,the expression of ITGB1 is increased.Both of them are associated with myometrial invasion,FIGO staging,lymph node metastasis and survival rate of patients with cervical squamous cell carcinoma.They are independent risk factors for adverse prognosis of patients with cervical squamous cell carcinoma.They may be used as markers for prognosis evaluation.