miR-335-5p和转录因子ATF2在宫颈癌中的表达关系研究

The Relevance Between the Expressions of MiR-335-5p and Transcription Factor ATF2 in Cervical Cancer

目的:探究微小RNA-335-5p(miR-335-5p)和激活转录因子2(ATF2)在宫颈癌中的表达,以及二者与宫颈癌患者临床病理特征的关系。方法:选取2017年8月至2019年10月在本院进行手术治疗的72例宫颈癌患者作为实验组,同期进行手术治疗的65例子宫肌瘤患者作为对照组,手术切除的宫颈癌癌组织和子宫肌瘤患者的宫颈组织作为研究所用样本。采用实时荧光定量PCR(qRT-PCR)检测miR-335-5p、ATF2 mRNA、基质金属蛋白酶2(MMP2)mRNA的表达;用免疫组化法检测ATF2和MMP2蛋白的阳性表达率;分析miR-335-5p、ATF2及MMP2表达与宫颈癌临床病理特征的关系;采用logistic多因素回归分析影响宫颈癌的因素;并分析miR-335-5p、ATF2及MMP2表达的相关性。结果:实验组中miR-335-5p、ATF2 mRNA及MMP2 mRNA表达水平均明显高于对照组(P<0.05);实验组ATF2和MMP2蛋白的阳性表达率均明显高于对照组(P<0.05);宫颈癌患者癌组织miR-335-5p、ATF2及MMP2的表达水平均与FIGO分期、浸润深度及淋巴结转移有关(P<0.05);Logistic多因素回归分析显示,miR-335-5p、ATF2及MMP2均是影响宫颈癌的危险因素;宫颈癌组织中miR-335-5p、ATF2和MMP2三者的表达量两两间均呈正相关(P<0.05)。结论:miR-335-5p、ATF2在宫颈癌组织中均呈高表达,二者与FIGO分期、浸润深度及淋巴结转移有关,且二者可能是影响宫颈癌的发生发展的生物学指标。

Objective:To investigate the expressions of microRNA-335-5p(miR-335-5p)and activated transcription factor 2(ATF2)in cervical cancer and their relationship with clinicopathological characteristics of cervical cancer patients.Methods:72 patients with cervical cancer who underwent surgical treatment in our hospital from August 2017 to October 2019 were selected as the experimental group,65 patients with uterine leiomyoma who underwent surgical treatment in the same period were selected as the control group,and cervical tissues of cervical cancer and uterine leiomyoma patients who underwent surgical resection were taken as the research samples.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the expression of miR-335-5p,ATF2 mRNA and matrix metalloproteinase 2(MMP2)mRNA;immunohistochemistry was used to detect the positive expression rate of ATF2 and MMP2 protein;the relationships between the expression of miR-335-5p,ATF2 and the clinicopathological characteristics of cervical cancer were analyzed;the factors affecting cervical cancer were analyzed by logistic regression analysis;and the correlation of expression of mir-335-5p,Atf2 and MMP2 was analyzed.Results:The expression levels of miR-335-5p,ATF2 mRNA and MMP2 mRNA in the experimental group were significantly higher than those in the control group(P<0.05).The positive expression rate of ATF2 and MMP2 protein in the experimental group was significantly higher than that in the control group(P<0.05).The expression levels of miR-335-5p,ATF2 and MMP2 in cervical cancer tissues were correlated with FIGO stage,depth of invasion and lymph node metastasis(P<0.05).Logistic multivariate regression analysis showed that miR-335-5p,ATF2 and MMP2 were risk factors for cervical cancer.There was a positive correlation between the expression of miR-335-5p,Atf2 and MMP2 in cervical cancer(P<0.05).Conclusion:The expressions of miR-335-5p and ATF2 are high in cervical cancer tissues.Both of them are related to FIGO stage,depth of invasion and lymph node metastasis,and they may be biological indicators affecting the occurrence and development of cervical cancer.